Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of ATP-dependent monoglucuronosyl bilirubin transport across rat canalicular membrane vesicles
Autore:
Kamisako, T; Leier, I;
Indirizzi:
Deutsch Krebsforschungszentrum, Div Tumor Biochem, D-69120 Heidelberg, Germany Deutsch Krebsforschungszentrum Heidelberg Germany D-69120 lberg, Germany
Titolo Testata:
HEPATOLOGY RESEARCH
fascicolo: 2, volume: 19, anno: 2001,
pagine: 103 - 107
SICI:
1386-6346(200102)19:2<103:COAMBT>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONJUGATED HYPERBILIRUBINEMIA; ANION TRANSPORT; MUTANT RATS; LIVER; DIGLUCURONIDE;
Keywords:
bilirubin; ATP-dependent transport; rat canalicular membrane vesicles; multidrug resistance protein 2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Kamisako, T Kinki Univ, Sch Med, Dept Gastroenterol & Hepatol, 377-2 Ohnohigashi, Osaka 5898511, Japan Kinki Univ 377-2 Ohnohigashi Osaka Japan 5898511 98511, Japan
Citazione:
T. Kamisako e I. Leier, "Characterization of ATP-dependent monoglucuronosyl bilirubin transport across rat canalicular membrane vesicles", HEPATOL RES, 19(2), 2001, pp. 103-107

Abstract

Bilirubin conjugates are secreted from hepatocytes into bile. Monoglucuronosyl bilirubin is an endogenous substrate for the multidrug resistance protein ?, which is located in rat hepatocyte canalicular membrane. We have characterized this ATP-dependent transport using rat canalicular membrane vesicles. Monoglucuronosyl bilirubin, H-3-labeled in the glucuronosyl moiety, was synthesized enzymatically using recombinant UDP-glycosyltransferase 1Al,and was stabilized with ascorbate. The rate for ATP-dependent transport ofmonoglucuronosyl bilirubin (at 0.5 muM) was 7.3 +/- 1.1 pmol mg protein(-1) min(-1) and the K-m value was 1.3 +/- 0.4 muM. This is the first time to demonstrate this kinetic constant of monoglucuronosyl bilirubin for the rathepatocyte canalicular membrane. The K-m value is similar to one for recombinant rat multidrug resistance protein 2. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 14:05:13