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Titolo:
Overexpression of cyclin D1 moderately increases ploidy in megakaryocytes
Autore:
Sun, S; Zimmet, JM; Toselli, P; Thompson, A; Jackson, CW; Ravid, K;
Indirizzi:
Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 h Med, Dept Biochem, Boston, MA 02118 USA Boston Univ, Sch Med, Dept Pharmacol, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 Med, Dept Pharmacol, Boston, MA 02118 USA Boston Univ, Sch Med, Whitaker Cardiovasc Inst, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 ker Cardiovasc Inst, Boston, MA 02118 USA St Jude Childrens Res Hosp, Div Expt Hematol, Memphis, TN 38105 USA St Jude Childrens Res Hosp Memphis TN USA 38105 ol, Memphis, TN 38105 USA
Titolo Testata:
HAEMATOLOGICA
fascicolo: 1, volume: 86, anno: 2001,
pagine: 17 - 23
SICI:
0390-6078(200101)86:1<17:OOCDMI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-MPL LIGAND; MYOBLAST DIFFERENTIATION; TRANSGENIC MICE; GROWTH-FACTOR; CELL-CYCLE; EXPRESSION; PROTEIN; CLONING; D3; IDENTIFICATION;
Keywords:
cyclin D1; megakaryocytes; ploidy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Ravid, K Boston Univ, Sch Med, Dept Biochem, K225,80 E Concord St, Boston,MA 02118USA Boston Univ K225,80 E Concord St Boston MA USA 02118 MA 02118USA
Citazione:
S. Sun et al., "Overexpression of cyclin D1 moderately increases ploidy in megakaryocytes", HAEMATOLOG, 86(1), 2001, pp. 17-23

Abstract

Background and Objectives. Megakalyocytes undergo a unique cell cycle by which they replicate their complete genome many times in the absence of cytokinesis, In the search for regulators of the endomitotic cell cycle, we previously produced mice transgenic for cyclin D3 to identify this cyclin as able to enhance ploidy and to increase the number of differentiated cells inthe megakaryocytic lineage. Of the D-type cyclins, cyclin D3 and to a muchlesser extent cyclin D1, are present in megakaryocytes undergoing endomitosis and these cyclins are, respectively, markedly and moderately upregulated following exposure to the ploidy-promoting factor, Mpl-ligand. Our objective was to explore whether cyclin D1 can mimic the effect of cyclin D3 on ploidy in megakalyocytes. Design and Methods. We generated transgenic mice overexpressing cyclin D1 in megakaryocytes and analyzed megakaryocyte ploidy, number and platelet levels in these mice and control mice. Results. We show that transgenic mice in which cyclin D1 is overexpressed in megakaryocytes display higher ploidy level than the control mice, with no change in the number of differentiated cells of the megakaryocytic series, or in platelet level. Interpretation and Conclusions. Our models support a key role for D-type cyclins in the endomitotic cell cycle, and also indicate that although cyclin D3, from among the D cyclins, is unique in its high levels of expression in megakaryocytes, it is not unique in its ability to promote polyploidization. (C) 2001, Ferrata Storti Foundation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 06:42:06