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Titolo:
Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene
Autore:
Chen, YH; Lee, WC; Liu, CF; Tsai, HJ;
Indirizzi:
Natl Taiwan Univ, Inst Fisheries Sci, Taipei 10764, Taiwan Natl Taiwan Univ Taipei Taiwan 10764 Fisheries Sci, Taipei 10764, Taiwan
Titolo Testata:
GENESIS
fascicolo: 1, volume: 29, anno: 2001,
pagine: 22 - 35
SICI:
1526-954X(200101)29:1<22:MSDEAP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE-SPECIFIC TRANSCRIPTION; MYOGENIC REGULATORY GENES; MOUSE EMBRYOGENESIS; MYOD TRANSCRIPTION; SOMITE FORMATION; PRECURSOR CELLS; SKELETAL-MUSCLE; EMBRYOS; FAMILY; MEMBER;
Keywords:
fish; microinjection; molecular structure; muscle-specificity; somitogenesis; transgenic fish;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Tsai, HJ 1 Roosevelt Rd,Sec 4, Taipei 106, Taiwan 1 Roosevelt Rd,Sec 4 Taipei Taiwan 106 ec 4, Taipei 106, Taiwan
Citazione:
Y.H. Chen et al., "Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene", GENESIS, 29(1), 2001, pp. 22-35

Abstract

We isolated a 1,438 bp cDNA fragment that encoded Myf-5 myogenic factor ofzebrafish. The deduced amino acid contained 237 residues, including the basic helix-loop-helix domain that is conserved in all known Myf-5. The zebrafish myf-5 transcripts were first detectable at 7.5 hpf, increased substantially until 16 hpf, and then declined gradually to an undetectable level by26 hpf. During somitogenesis, zebrafish myf-5 transcripts were distributedmainly in the somites and segmental plates. Prominent signals occurred transiently in adaxial cells in two parallel rows but did not extend beyond the positive-signal somites. Various lengths of upstream region of zebrafish myf-5 fused with EGFP gene were used to carry out transgenic analysis. Results showed that a small, 82 bp (nucleotide positions from -82 to -1), regulatory cassette is sufficient to control the somite- and stage-specific expression of zebrafish myf-5 during early development. genesis 29:22-35, 2001. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:55:54