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Titolo:
6-formylpterin, a xanthine oxidase inhibitor, intracellularly generates reactive oxygen species involved in apoptosis and cell proliferation
Autore:
Arai, T; Endo, N; Yamashita, K; Sasada, M; Mori, H; Ishii, H; Hirota, K; Makino, K; Fukuda, K;
Indirizzi:
Kyoto Univ Hosp, Dept Anesthesia, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Hosp Kyoto Japan 6068507 esia, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ, Inst Adv Energy, Uji, Kyoto, Japan Kyoto Univ Uji Kyoto Japan yoto Univ, Inst Adv Energy, Uji, Kyoto, Japan Kyoto Univ Hosp, Dept Hematol & Oncol, Kyoto 606, Japan Kyoto Univ Hosp Kyoto Japan 606 , Dept Hematol & Oncol, Kyoto 606, Japan Kyoto Univ, Coll Med Technol, Kyoto 606, Japan Kyoto Univ Kyoto Japan 606 yoto Univ, Coll Med Technol, Kyoto 606, Japan
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 3, volume: 30, anno: 2001,
pagine: 248 - 259
SICI:
0891-5849(20010201)30:3<248:6AXOII>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELAYED NEURONAL DEATH; HYDROGEN-PEROXIDE PRODUCTION; SUPEROXIDE ANION; FAS LIGAND; ISCHEMIA; HIPPOCAMPUS; PTERIN-6-ALDEHYDE; PROTEASE; GROWTH; H2O2;
Keywords:
6-formylpterin; conjugated pterin; NADH; reactive oxygen species; apoptosis; cell proliferation; Fas;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Arai, T Kyoto Univ Hosp, Dept Anesthesia, Sakyo Ku, 54 Kawahara Cho, Kyoto6068507, Japan Kyoto Univ Hosp 54 Kawahara Cho Kyoto Japan 6068507 068507, Japan
Citazione:
T. Arai et al., "6-formylpterin, a xanthine oxidase inhibitor, intracellularly generates reactive oxygen species involved in apoptosis and cell proliferation", FREE RAD B, 30(3), 2001, pp. 248-259

Abstract

The chemical property of 6-formylpterin and its biological functions were examined. Polarographic studies revealed that 6-formylpterin reacted with NAD(P)H and consumed oxygen. In contrast, other conjugated pterins, such as biopterin and neopterin, showed no consumption of oxygen. The production analysis using high-performance liquid chromatography documented that 6-formylpterin catalyzes the conversion from NADH to NAD. Electroparamagnetic resonance spin trapping experiments demonstrated that this reaction is accompanied with the generation of reactive oxygen species (ROS), superoxide anion and hydrogen peroxide, When 6-formylpterin was administered to HL-60 cells,intracellular ROS generation was observed and apoptosis was induced. In contrast, other conjugated pterins induced neither intracellular ROS generation nor apoptosis in HL-60 cells. The intracellular ROS generation by 6-formylpterin was observed in other cells, such as PanC-1 cells and Jurkat cells. 6-formylpterin suppressed cell proliferation in PanC-1 cells and inhibited Fas-mediated apoptosis in Jurkat cells. These findings indicate that, among conjugated pterins, 6-formylpterin has the unique property to transfer electron from NAD(P)H to oxygen and that the property brings about intracellular ROS generation, which exerts various biological functions such as induction of apoptosis, suppression of cell proliferation, and inhibition of Fas-mediated apoptosis. (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:51:19