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Titolo:
The role of pyridoxal phosphate in the function of EspB, a protein secreted by enteropathogenic Escherichia coli
Autore:
Taylor, KA; OConnell, CB; Thompson, R; Donnenberg, MS;
Indirizzi:
Univ Maryland, Sch Med, Dept Med, Div Infect Dis, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Infect Dis, Baltimore, MD 21201 USA Univ Maryland, Sch Med, Dept Microbiol & Immunol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 l & Immunol, Baltimore, MD 21201 USA Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 & Mol Biol, Baltimore, MD 21201 USA
Titolo Testata:
FEBS LETTERS
fascicolo: 1-2, volume: 488, anno: 2001,
pagine: 55 - 58
SICI:
0014-5793(20010112)488:1-2<55:TROPPI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOST EPITHELIAL-CELLS; PATHOGENICITY ISLAND; HELA-CELLS; MONOLAYERS; ATTACHMENT; ADHESION;
Keywords:
protein secretion; pyridoxal phosphate; bacterial pathogenesis; fluorometry; enteropathogenic Escherichia coli;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Donnenberg, MS Univ Maryland, Sch Med, Dept Med, Div Infect Dis, 10 S PineSt,MSTF-900, Baltimore, MD 21201 USA Univ Maryland 10 S Pine St,MSTF-900 Baltimore MD USA 21201
Citazione:
K.A. Taylor et al., "The role of pyridoxal phosphate in the function of EspB, a protein secreted by enteropathogenic Escherichia coli", FEBS LETTER, 488(1-2), 2001, pp. 55-58

Abstract

The sequence of EspB, a secreted protein required for virulence of enteropathogenic Escherichia coli (EPEC), reveals a motif common to enzymes that bind pyridoxal phosphate. Pyridoxal phosphate was not found by fluorometry in concentrated supernatants of EPEC cultures that contain EspB. Plasmids containing cloned espB, in which the lysine residue conserved in the motif was substituted with either an arginine or methionine residue, remained capable of complementing an espB deletion mutant to restore EspB function. The results of these studies do not support a role for pyridoxal phosphate in EspB function. (C) 2001 federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/08/20 alle ore 23:11:28