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Titolo:
Novel type Arabidopsis thaliana H+-PPase is localized to the Golgi apparatus
Autore:
Mitsuda, N; Enami, K; Nakata, M; Takeyasu, K; Sato, MH;
Indirizzi:
Kyoto Univ, Fac Integrated Human Studies, Dept Nat Environm Sci, Sakyo Ku,Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 vironm Sci, Sakyo Ku,Kyoto 6068501, Japan Kyoto Univ, Grad Sch Human & Environm Studies, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 m Studies, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ, Grad Sch Biostudies, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 iostudies, Sakyo Ku, Kyoto 6068501, Japan
Titolo Testata:
FEBS LETTERS
fascicolo: 1-2, volume: 488, anno: 2001,
pagine: 29 - 33
SICI:
0014-5793(20010112)488:1-2<29:NTATHI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; INORGANIC PYROPHOSPHATASE; TONOPLAST VESICLES; CORN COLEOPTILES; BREFELDIN-A; CELLS; MEMBRANE; MITOCHONDRIA; ATPASE; PLANTS;
Keywords:
AVP2/AVPL1 gene; Golgi apparatus; green fluorescent protein; brefeldin A; H+-PPase; Arabidopsis thaliana;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sato, MH Kyoto Univ, Fac Integrated Human Studies, Dept Nat Environm Sci, Sakyo Ku,Yoshida Nihonmatsu Cho, Kyoto 6068501, Japan Kyoto Univ Yoshida Nihonmatsu Cho Kyoto Japan 6068501 501, Japan
Citazione:
N. Mitsuda et al., "Novel type Arabidopsis thaliana H+-PPase is localized to the Golgi apparatus", FEBS LETTER, 488(1-2), 2001, pp. 29-33

Abstract

Vacuolar H+-PPase, a membrane bound proton-translocating pyrophosphatase found in various species including plants, some protozoan and prokaryotes, has been demonstrated to be localized to the vacuolar membrane in plants. Using a GUS reporter system and a green fluorescent protein (GFP) fusion protein, we investigated the tissue distribution and the subcellular localization, respectively, of a novel type H+-PPase encoded by AVP2/AVPL1 identifiedin the Arabidopsis thaliana genome. We showed that AVP2/AVPL1 is highly expressed at the trichoma and the filament of stamen. Furthermore, the fluorescence of GFP-tagged AVP2/AVPL1 shelved small dotlike structures that were observed throughout the cytoplasm of various Arabidopsis cells under a fluorescent microscope. The distribution of this dot-like fluorescent pattern was apparently affected by a treatment with brefeldin A. Moreover, we demonstrated that most dot-like fluorescent structures colocalized with a Golgi resident protein. These findings suggest that this novel type H+-PPase resides on the Golgi apparatus rather than the vacuolar membrane. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 09:49:39