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Titolo:
Retinoid X receptor alpha and retinoic acid receptor gamma mediate expression of genes encoding tight-junction proteins and barrier function in F9 cells during visceral endodermal differentiation
Autore:
Kubota, H; Chiba, H; Takakuwa, Y; Osanai, M; Tobioka, H; Kohama, GI; Mori, M; Sawada, N;
Indirizzi:
Sapporo Med Univ, Sch Med, Dept Pathol, Chuo Ku, Sapporo, Hokkaido 0608556, Japan Sapporo Med Univ Sapporo Hokkaido Japan 0608556 , Hokkaido 0608556, Japan Sapporo Med Univ, Sch Med, Dept Oral Surg, Sapporo, Hokkaido 0608556, Japan Sapporo Med Univ Sapporo Hokkaido Japan 0608556 , Hokkaido 0608556, Japan
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 263, anno: 2001,
pagine: 163 - 172
SICI:
0014-4827(20010201)263:1<163:RXRAAR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYONAL CARCINOMA-CELLS; TERATOCARCINOMA CELLS; MOUSE; HETERODIMERS; OCCLUDIN; GASTRULATION; SEQUENCE; CLONING; CLAUDIN; STRANDS;
Keywords:
RAR; RXR; retinoids; tight junctions; F9 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Chiba, H Sapporo Med Univ, Sch Med, Dept Pathol, Chuo Ku, South 1,West 17,Sapporo,Hokkaido 0608556, Japan Sapporo Med Univ South 1,West 17 Sapporo Hokkaido Japan 0608556 n
Citazione:
H. Kubota et al., "Retinoid X receptor alpha and retinoic acid receptor gamma mediate expression of genes encoding tight-junction proteins and barrier function in F9 cells during visceral endodermal differentiation", EXP CELL RE, 263(1), 2001, pp. 163-172

Abstract

Retinoids are critical for differentiation of columnar epithelial cells and for preventing metaplasia of these cells into stratified squamous epithelial cells, in which tight junctions (TJs) are essentially absent. This implies that retinoids might play important roles in regulating the structures and functions of TJs of columnar epithelium. F9 murine embryonal carcinoma cells differentiate into epithelial cells resembling visceral endoderm bearing TJs, when grown in suspension as aggregates in the presence of retinoicacid (RA). We show that RA induces the TJ structure and expression of several TJ-associated molecules, such as ZO-1, occludin, claudin-6, and claudin-7, as well as a barrier function in the genetically engineered cell line F9: rtTA:Cre-ERT L32T2, which allows sophisticated genetic manipulations simply by addition of ligands (H. Chiba et at, 2000, Exp. Cell Res. 260, 334-339). Interestingly, our data indicate that a barrier for small substances is generated after that for large ones during de novo formation of TJs, We also compared the RA-induced expression of TJ components and barrier function in RXR alpha (-/-)-RAR gamma (-/-) F9 cells with those in wild-type cellsand show that the retinoid signals for transduction of these events are mediated by specific RXR-RAR pairs. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:56:19