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Titolo:
Cloning and characterization of P110, a novel small nucleolar U3 ribonucleoprotein, expressed in early development
Autore:
Adamson, C; Niu, S; Bahl, JJ; Morkin, E;
Indirizzi:
Univ Arizona, Arizona Hlth Sci Ctr, Sarver Heart Ctr, Tucson, AZ 85724 USAUniv Arizona Tucson AZ USA 85724 , Sarver Heart Ctr, Tucson, AZ 85724 USA Univ Arizona, Dept Med, Tucson, AZ USA Univ Arizona Tucson AZ USAUniv Arizona, Dept Med, Tucson, AZ USA
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 1, volume: 263, anno: 2001,
pagine: 55 - 64
SICI:
0014-4827(20010201)263:1<55:CACOPA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; NUCLEOPLASMIN FAMILY; PROTEIN-STRUCTURE; NUCLEAR-PROTEIN; RNA RECOGNITION; BINDING PROTEIN; SPLICING FACTOR; GLUTAMINE-RICH; CDNA CLONING; LOCALIZATION;
Keywords:
nucleolus; glutamine-rich protein; chick embryo; U3 snoRNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Morkin, E Univ Arizona, Arizona Hlth Sci Ctr, Sarver Heart Ctr, 1501 N Campbell Ave,Tucson, AZ 85724 USA Univ Arizona 1501 N Campbell Ave Tucson AZ USA 85724 Z 85724 USA
Citazione:
C. Adamson et al., "Cloning and characterization of P110, a novel small nucleolar U3 ribonucleoprotein, expressed in early development", EXP CELL RE, 263(1), 2001, pp. 55-64

Abstract

We report the cloning of a BrdU-sensitive transcript of 4.1 kb from an immortalized quail heart cell line containing an open reading frame of 940 amino acids (110 kDa, pI similar to 5.18). The mRNA encoding P110 appears in the heart and neural tube by 36 h of avian development, at a time when theseorgans are rapidly developing. Analysis of the DNA-deduced protein sequence revealed a bipartite nuclear localization signal, and a highly charged domain rich in both acidic and basic residues, Immunofluorescent staining with polyclonal antibodies raised against a P110 peptide localized the proteinto the nucleolus of avian and mammalian cells. Although database search showed significant homology with an uncharacterized cDNA from human brain andseveral human and mouse Expressed Sequence Tags, there was no close homology to known nucleolar proteins. Immunoprecipitation of P110 from cell sonicates revealed it contained U3 small nucleolar RNA but no significant amounts of other box C/D small nucleolar RNAs. These data suggest that P110 is one of the U3 small nucleolar ribonucleoproteins that are involved in rRNA processing. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 15:03:47