Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Spindle formation in karyogamy-blocked zygotes of the isogamous brown algaScytosiphon lomentaria (Scytosiphonales, Phaeophyceae)
Autore:
Nagasato, C; Motomura, T; Ichimura, T;
Indirizzi:
Hokkaido Univ, Fac Sci, Inst Algolog Res, Muroran, Hokkaido 0510003, JapanHokkaido Univ Muroran Hokkaido Japan 0510003 ran, Hokkaido 0510003, Japan
Titolo Testata:
EUROPEAN JOURNAL OF PHYCOLOGY
fascicolo: 4, volume: 35, anno: 2000,
pagine: 339 - 347
SICI:
0967-0262(200011)35:4<339:SFIKZO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUCUS-DISTICHUS FUCALES; LAMINARIA-ANGUSTATA PHAEOPHYTA; DICTYOTA-DICHOTOMA PHAEOPHYTA; SEA-URCHIN EGGS; IMMUNOFLUORESCENCE MICROSCOPY; THALLUS DEVELOPMENT; CELL-CYCLE; FERTILIZATION; ELECTRON; CENTROSOME;
Keywords:
centrosome; isogamy; karyogamy block; Scytosiphon lomentaria; spindle formation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Nagasato, C Hokkaido Univ, Fac Sci, Inst Algolog Res, Muroran, Hokkaido 0510003, Japan Hokkaido Univ Muroran Hokkaido Japan 0510003 o 0510003, Japan
Citazione:
C. Nagasato et al., "Spindle formation in karyogamy-blocked zygotes of the isogamous brown algaScytosiphon lomentaria (Scytosiphonales, Phaeophyceae)", EUR J PHYC, 35(4), 2000, pp. 339-347

Abstract

The first spindle formation in karyogamy-blocked zygotes in the isogamous brown alga Scytosiphon lomentaria was studied by electron and immunofluorescence microscopy using anti-centrin and anti-beta -tubulin antibodies. Zygotes blocked in karyogamy by incubation at high temperature (22 degreesC) were compared with normal zygotes incubated at 14 degreesC. In normal zygotes, after the disappearance of female gamete centrioles. centrioles from the male gamete duplicated and migrated to both poles in the metaphase, and a bipolar spindle was formed. The nuclear envelope was almost intact except atthe poles. In karyogamy-blocked zygotes of S. lomentaria, both DNA synthesis and chromosomal condensation occurred in the male and female nuclei. A bipolar mitotic spindle with a pair of centrioles at each of two poles couldbe observed in the male nucleus, but not in the female nucleus. In some cases, after a pair of centrioles derived from the male gamete duplicated andseparated, one of the two pairs migrated towards the female nucleus. In this case, a monopolar spindle was formed independently in each of the femaleand male nuclei, In addition to the monopolar spindle, a bipolar spindle was formed lying across the unfused male and female nuclei. Male and female chromosomes were intermixed and some of them were incorporated into the spindle. After cytokinesis of the normal and karyogamy-blocked zygotes, two daughter cells, each with one nucleus and one chloroplast, were formed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:54:30