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Titolo:
Isolation and characterization of a chromium-reducing bacterium from a chromated copper arsenate-contaminated site
Autore:
McLean, JS; Beveridge, TJ; Phipps, D;
Indirizzi:
Univ Guelph, Coll Biol Sci, Dept Microbiol, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 icrobiol, Guelph, ON N1G 2W1, Canada Total Forest Ind Ltd, Guelph, ON N21 1ES, Canada Total Forest Ind Ltd Guelph ON Canada N21 1ES Guelph, ON N21 1ES, Canada
Titolo Testata:
ENVIRONMENTAL MICROBIOLOGY
fascicolo: 6, volume: 2, anno: 2000,
pagine: 611 - 619
SICI:
1462-2912(200012)2:6<611:IACOAC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEXAVALENT-CHROMIUM; ENTEROBACTER-CLOACAE; ENZYMATIC REDUCTION; STRAIN; CR(VI); CULTURES; LB300;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Beveridge, TJ Univ Guelph, Coll Biol Sci, Dept Microbiol, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 ph, ON N1G 2W1, Canada
Citazione:
J.S. McLean et al., "Isolation and characterization of a chromium-reducing bacterium from a chromated copper arsenate-contaminated site", ENVIRON MIC, 2(6), 2000, pp. 611-619

Abstract

A Gram-negative bacterium (CRB5) was isolated from a chromium-contaminatedsite that was capable of reducing hexavalent chromium to an insoluble precipitate, thereby removing this toxic chromium species from solution. Analysis of the 16S rRNA from the isolate revealed that it was a pseudomonad withhigh similarity to Pseudomaonas synxantha. CRB5 was tolerant to high concentrations of chromate (500 mg l(-1)) and can reduce Cr(VI) under aerobic and anaerobic conditions. It also exhibited a broad range of reduction efficiencies under minimal nutrient conditions at temperatures between 4 degreesCand 37 degreesC and at pH levels from 4 to 9. As reduction increased, so did total cellular protein, indicating that cell growth was a requirement for reduction. Under low nutrient conditions with CRB5 or when using nonsterile contaminated groundwater from the site, reduction of Cr(VI) was followedby a increase in solution turbidity as a result of the formation of fine-grained Cr(III) precipitates, most probably chromium hydroxide mineral phases such as Cr(OH)(3). Chromium adsorption and precipitation, as observed by transmission electron microscopy coupled with energy dispersive X-ray spectroscopy (TEM/EDS), revealed that the surfaces of the cells were uniformly stained with bound Cr(III) and amorphous precipitates (as determined by selected area electron diffraction; SAED). A mass balance of chromium in a batch bioreactor revealed that up to 30% of the total Cr was as settable precipitates or bound to cells.

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Documento generato il 09/07/20 alle ore 01:41:53