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Titolo:
In situ hybridization detection of single-copy human papillomavirus on isolated cells, using a catalyzed signal amplification system: GenPoint (TM)
Autore:
Lizard, G; Demares-Poulet, MJ; Roignot, P; Gambert, P;
Indirizzi:
CHU Dijon, Hop Bocage, Inserm U498, Lab Biochim Med, F-21034 Dijon, FranceCHU Dijon Dijon France F-21034 8, Lab Biochim Med, F-21034 Dijon, France
Titolo Testata:
DIAGNOSTIC CYTOPATHOLOGY
fascicolo: 2, volume: 24, anno: 2001,
pagine: 112 - 116
SICI:
8755-1039(200102)24:2<112:ISHDOS>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; REPORTER DEPOSITION; CONFOCAL MICROSCOPY; DNA; CARCINOMA; SEQUENCES; IMMUNOHISTOCHEMISTRY; LESIONS; CERVIX; INSITU;
Keywords:
papillomavirus; in situ hybridization; catalyzed signal amplification system; GenPoint (TM);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Lizard, G CHU Dijon, Hop Bocage, Inserm U498, Lab Biochim Med, BP 1542, F-21034 Dijon, France CHU Dijon BP 1542 Dijon France F-21034 2, F-21034 Dijon, France
Citazione:
G. Lizard et al., "In situ hybridization detection of single-copy human papillomavirus on isolated cells, using a catalyzed signal amplification system: GenPoint (TM)", DIAGN CYTOP, 24(2), 2001, pp. 112-116

Abstract

The performance and drawbacks of GenPoint(TM), which is a catalyzed signalamplification system for immunohistochemistry, have been evaluated for itsability to reveal human papillomavirus (HPV) DNA detected by in situ hybridization with biotinylated DNA probes. For this aim, formalin-fixed cell deposits from carcinoma cells of the uterine cervix, CaSki, SiHa, and HeLa, containing, respectively, 600 copies of HPV DNA type 16, 1-2 copies of HPV DNA type 16, and 10-50 copies of HPV DNA type 18, were used, and the GenPoint(TM) method (consisting of successive incubations with peroxidase-conjugated streptavidin, biotinyl tyramide, and peroxidase-conjugated streptavidin)was compared to immunoenzymatic revelation procedures involving either a one-step reaction (streptavidin-alkaline phosphatase or streptavidin-peroxidase), or a three-step reaction (anti-biotin mouse monoclonal antibody, rabbit anti-mouse antiserum, and mouse APAAP complex). In these conditions, after analysis with a bright-field microscope. GenPoint(TM) appeared the most sensitive method of revelation, easily allowing detection of 1-2 copies of HPV DNA on isolated cells by in situ hybridization. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:49:38