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Titolo:
Macrophage activation in type 1 diabetic patients with catheter obstruction during peritoneal insulin delivery with an implantable pump
Autore:
Kessler, L; Tritschler, S; Bohbot, A; Sigrist, S; Karsten, V; Boivin, S; Dufour, P; Belcourt, A; Pinget, M;
Indirizzi:
Hop Univ, Dept Endocrinol Diabetol, Serv Endocrinol Diabetol, Praticien Hosp, F-67091 Strasbourg, France Hop Univ Strasbourg France F-67091 cien Hosp, F-67091 Strasbourg, France Hautepierre Hosp, European Ctr Study Diabet, Strasbourg, France Hautepierre Hosp Strasbourg France Ctr Study Diabet, Strasbourg, France Hautepierre Hosp, Dept Hematol, Strasbourg, France Hautepierre Hosp Strasbourg France sp, Dept Hematol, Strasbourg, France
Titolo Testata:
DIABETES CARE
fascicolo: 2, volume: 24, anno: 2001,
pagine: 302 - 307
SICI:
0149-5992(200102)24:2<302:MAIT1D>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFUSION; THERAPY; IDDM; IMMUNOGENICITY; MULTICENTER; FREQUENCY; SYSTEMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Kessler, L Hop Univ, Dept Endocrinol Diabetol, Serv Endocrinol Diabetol, Praticien Hosp, 1 Pl Hop, F-67091 Strasbourg, France Hop Univ 1 Pl Hop Strasbourg France F-67091 Strasbourg, France
Citazione:
L. Kessler et al., "Macrophage activation in type 1 diabetic patients with catheter obstruction during peritoneal insulin delivery with an implantable pump", DIABET CARE, 24(2), 2001, pp. 302-307

Abstract

OBJECTIVE- The purpose of this study was to evaluate the activation of macrophages in type 1 diabetic patients during peritoneal insulin delivery with an implantable pump against two types of insulin: that which was collected from the pump reservoir and that which came straight from the bottle (i.e., vial insulin). Macrophage activation was studied in patients with and without catheter obstruction and compared with activation in healthy subjects. RESEARCH DESIGN AND METHODS- Human insulin (21PH, 400 U/ml; Hoescht) was collected from the pump reservoir (Minimed) of diabetic patients with (n - 3) or without (n = 7) catheter obstruction, as assessed by histological examination of the catheter tip. Monocytes were obtained from venous blood samples from both kinds of diabetic patients and from healthy subjects (n = 5) and were differentiated into monocyte-derived macrophages in culture. Theirchemotaxis and tumor necrosis factor-alpha (TNF-alpha) release were studied with respect to bath types of insulin, as previously stated. Formyl-methionyl-leucyl-phenylalanine (fMLP) and lipopolysaccharide (LPS) were used as controls. RESULTS- Neither insulin recovered from the pump reservoir nor vial insulin proved chemotactic to macrophages from either healthy subjects or those diabetic patients with and without catheter obstruction. The migration toward fMLP of macrophages from patients presenting a catheter obstruction was significantly higher than that observed with macrophages from tither diabetic patients without obstruction or healthy subjects; the chemotactic index (mean +/- SD) was 3.81 +/- 0.36 vs. 2.30 +/- 0.89 and 2.60 +/- 0.80, respectively (P < 0.05). LPS significantly stimulated the TNF-<alpha> secretion ofmacrophages from diabetic subjects with a catheter obstruction, whereas both native and reservoir-recovered insulin had no effect on this release (144.83 +/- 67.25 vs. 5.15 +/- 2.93 and 5.27 +/- 2.43 pg/ml, P < 0.001). CONCLUSIONS- The human insulin used in implantable pumps, regardless of how long it had remained in the pump reservoir, did not induce macrophage activation in diabetic patients treated through intraperitoneal insulin delivery. In some of these diabetic patients, catheter obstruction could he explained by their high capacity of macrophage chemotaxis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:29:13