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Titolo:
Percutaneous endocardial transfer and expression of genes to the myocardium utilizing fluoroscopic guidance
Autore:
Sanborn, TA; Hackett, NR; Lee, LY; El-Sawy, T; Blanco, I; Tarazona, N; Deutsch, E; Crystal, RG; Rosengart, TK;
Indirizzi:
Cornell Univ, New York Presbyterian Hosp, Weill Med Coll, Div Cardiol, NewYork, NY USA Cornell Univ New York NY USA eill Med Coll, Div Cardiol, NewYork, NY USA Cornell Univ, New York Presbyterian Hosp, Weill Med Coll, Div Pulm & Crit Care Med, New York, NY USA Cornell Univ New York NY USA Div Pulm & Crit Care Med, New York, NY USA Cornell Univ, New York Presbyterian Hosp, Weill Med Coll, Belfer Gene Therapy Core Facil, New York, NY USA Cornell Univ New York NY USA r Gene Therapy Core Facil, New York, NY USA Evanston Northwestern Healthcare, Div Cardiothorac Surg, Evanston, IL 60201 USA Evanston Northwestern Healthcare Evanston IL USA 60201 ston, IL 60201 USA Cornell Univ, New York Presbyterian Hosp, Weill Med Coll, Inst Med Genet, New York, NY USA Cornell Univ New York NY USA Med Coll, Inst Med Genet, New York, NY USA
Titolo Testata:
CATHETERIZATION AND CARDIOVASCULAR INTERVENTIONS
fascicolo: 2, volume: 52, anno: 2001,
pagine: 260 - 266
SICI:
1522-1946(200102)52:2<260:PETAEO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISCHEMIC MYOCARDIUM; CYSTIC-FIBROSIS; HEART-DISEASE; GROWTH-FACTOR; BLOOD-FLOW; IN-VIVO; ADENOVIRUS; ANGIOGENESIS; INDIVIDUALS; INJECTION;
Keywords:
gene therapy; catheter; fluoroscopy; myocardium; angiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Sanborn, TA Evanston Northwestern Healthcare, Div Cardiol, 2650 Ridge Ave,Burch 300, Evanston, IL 60201 USA Evanston Northwestern Healthcare 2650 Ridge Ave,Burch 300 Evanston IL USA 60201
Citazione:
T.A. Sanborn et al., "Percutaneous endocardial transfer and expression of genes to the myocardium utilizing fluoroscopic guidance", CATHET C IN, 52(2), 2001, pp. 260-266

Abstract

Experimental studies indicate that administration of angiogenic proteins or genes by the epicardial or intracoronary route can stimulate development of new collateral vessels and improve myocardial perfusion. An endocardial catheter-based approach to this therapy would obviate the need for surgery,while preserving the effectiveness of direct intramyocardial administration. Fluoroscopic guidance and prototype, preformed, coaxial catheters were used to examine the feasibility of percutaneous catheter-based adenovirus (Ad)-mediated gene transfer and expression in normal swine myocardium. The feasibility of intramyocardial administration (100 mul/injection) of a radiocontrast agent and black tissue dye to all regions of the left ventricle (septum, anterior, lateral, and inferior wall) was confirmed fluoroscopically and on postmortem examination. Injections of replication-deficient adenovirus (10 injections of 10(11) particle units/100 mul each) coding for beta -galactosidase (Ad beta gal) or vascular endothelial growth factor (Ad(GV)VEGF121.10) were administered to the left ventricular free wall to examine endocardial based gene transfer and expression. beta -Galactosidase activity was detected by histochemical staining and quantitative assay in targeted regions of the myocardium. Regional VEGF expression was found to be significantly greater in targeted regions (1.3 +/- 0.4 ng/mg protein) as compared with non-targeted regions (0.3 +/- 0.1 ng/mg protein) or regions injected with control (Ad beta gal) virus (0.2 +/- 0.03 ng/mg protein, P < 0.001). Catheter-based Ad mediated endocardial gene transfer and expression is feasibleusing percutaneous, fluoroscopically guided, preformed, coaxial catheters. This approach should be clinically useful to administer angiogenic genes to the ischemic myocardium. Cathet Cardiovasc Intervent 2001;52:260-266. (C)2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 03:12:56