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Titolo:
Formation of abasic sites in DNA by t-butyl peroxyl radicals: implication for potent genotoxicity of lipid peroxyl radicals
Autore:
Kanazawa, A; Sawa, T; Akaik, T; Maeda, H;
Indirizzi:
Kumamoto Univ, Sch Med, Dept Microbiol, Kumamoto 8600811, Japan Kumamoto Univ Kumamoto Japan 8600811 Microbiol, Kumamoto 8600811, Japan Kumamoto Univ, Fac Educ, Kumamoto 8608555, Japan Kumamoto Univ Kumamoto Japan 8608555 , Fac Educ, Kumamoto 8608555, Japan
Titolo Testata:
CANCER LETTERS
fascicolo: 1, volume: 156, anno: 2000,
pagine: 51 - 55
SICI:
0304-3835(20000801)156:1<51:FOASID>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEME-IRON; MUTATIONS; ADDUCTS; TRANSVERSIONS; PEROXIDATION; MUTAGENESIS; DAMAGE; CANCER; WOMEN; MEAT;
Keywords:
alkyl peroxyl radical; abasic site; DNA modification;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Maeda, H Kumamoto Univ, Sch Med, Dept Microbiol, Honjo 2-2-1, Kumamoto 8600811, Japan Kumamoto Univ Honjo 2-2-1 Kumamoto Japan 8600811 8600811, Japan
Citazione:
A. Kanazawa et al., "Formation of abasic sites in DNA by t-butyl peroxyl radicals: implication for potent genotoxicity of lipid peroxyl radicals", CANCER LETT, 156(1), 2000, pp. 51-55

Abstract

We investigated abasic site formation in calf thymus DNA after exposure toa model compound of lipid-derived peroxyl radical that was generated by the reaction of tert-butyl hydroperoxide (t-BuOOH) with hemoglobin Abasic site density in DNA was quantified by use of an enzyme-linked immunosorvent assay-like assay. in the presence of 10 mM t-BuOOH and 12.5 or 25 muM hemoglobin, 0.6-1.0 abasic sites/10(4) nucleotides were formed. However, abasic sites were not detected after replacing hemoglobin with nonheme iron, e.g. EDTA/Fe2+, which initiates the production of alkyl and alkoxyl radicals. Therefore, the present results suggest that lipid peroxyl radicals may have a genotoxic potential through unique reactions, including depurination and depyrimidination, which lead to DNA strand breakage. (C) 2000 Elsevier ScienceIreland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 02:00:16