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Titolo:
Nociceptin/orphanin FQ and [Phe(1)psi(CH2-NH)Gly(2)]nociceptin(1-13)NH2 modulates the activity of hypothalamic paraventricular nucleus neurons in vitro
Autore:
Shirasaka, T; Miyahara, S; Takasaki, M; Kannan, H;
Indirizzi:
Miyazaki Med Coll, Dept Anesthesiol, Miyazaki 8891692, Japan Miyazaki Med Coll Miyazaki Japan 8891692 hesiol, Miyazaki 8891692, Japan Miyazaki Med Coll, Dept Physiol 1, Miyazaki 8891692, Japan Miyazaki Med Coll Miyazaki Japan 8891692 siol 1, Miyazaki 8891692, Japan Miyazaki Med Coll, Dept Neurosurg, Miyazaki 8891692, Japan Miyazaki Med Coll Miyazaki Japan 8891692 rosurg, Miyazaki 8891692, Japan
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1, volume: 890, anno: 2001,
pagine: 147 - 153
SICI:
0006-8993(20010126)890:1<147:NFA[M>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CA2+ CHANNEL CURRENT; ORPHANIN-FQ/NOCICEPTIN; IN-VITRO; CONSCIOUS RATS; SPINAL-CORD; RECEPTOR; INHIBITION; AGONIST; RELEASE; TRANSMISSION;
Keywords:
nociceptin; nocistatin; [Phe(1)psi(CH2-NH)Gly(2)]nociceptin(1-13)NH2 paraventricular nucleus; hyperpolarization; potassium current;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Kannan, H Miyazaki Med Coll, Dept Anesthesiol, 5200 Kihara, Miyazaki 8891692, Japan Miyazaki Med Coll 5200 Kihara Miyazaki Japan 8891692 692, Japan
Citazione:
T. Shirasaka et al., "Nociceptin/orphanin FQ and [Phe(1)psi(CH2-NH)Gly(2)]nociceptin(1-13)NH2 modulates the activity of hypothalamic paraventricular nucleus neurons in vitro", BRAIN RES, 890(1), 2001, pp. 147-153

Abstract

Nociceptin, also known as orphanin FQ (N/OFQ), an endogenous ligand for the orphan opioid receptor-like(1) (ORL1) receptor, is moderately expressed in the hypothalamic paraventricular nucleus (PVN) involved in the integrative control of the function of the endocrine and autonomic nervous systems. Our previous study demonstrated that intracerebroventricular administration of N/OFQ elicits an inhibitor, action on the function of the cardiovascularand sympathetic nervous systems in conscious rats. However, the effects ofN/OFQ on PVN neurons have not been examined. We investigated the effects of N/OFQ on PVN neurons using a whole-cell patch-clamp recording technique in rat brain slices. N/OFQ (30-1000 nM) hyperpolarized membrane potentials in type 1 and type 2 neurons of the PVN classified by the electrophysiological property. [Phe(1)psi (CH2-NH)Gly(2)]nociceptin(1-13)NH2 (Phe psi) (1-9 muM), a presumed competitive antagonist of the ORL1 receptor, also hyperpolarized membrane potential in both types of neurons. In voltage clamp studies, N/OFQ (3-3000 nM) activated a K+ current concentration-dependently in 69.7% of PVN neurons with an EC50 of 72.4+/-12 nM. Phe psi (100-9000 nM) also activated a K+ current with an EC,, of 818+/-162 nM in PVN neurons, and significantly reduced the amplitude of the N/OFQ-stimulated current. The N/OFQ-induced current was not antagonized by the classical opioid receptor antagonist naloxone and putative antagonist nocistatin. These findings suggest that N/OFQ may have a functional role in the PVN. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 09:49:20