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Titolo:
Glucose-induced inhibition of in vitro bone mineralization
Autore:
Balint, E; Szabo, P; Marshall, CF; Sprague, SM;
Indirizzi:
Northwestern Univ, Div Nephrol, Evanston NW Healthcare, Sch Med,Dept Med, Evanston, IL 60201 USA Northwestern Univ Evanston IL USA 60201 ,Dept Med, Evanston, IL 60201 USA Northwestern Univ, Res Inst, Evanston NW Healthcare, Sch Med, Evanston, IL60201 USA Northwestern Univ Evanston IL USA 60201 e, Sch Med, Evanston, IL60201 USA
Titolo Testata:
BONE
fascicolo: 1, volume: 28, anno: 2001,
pagine: 21 - 28
SICI:
8756-3282(200101)28:1<21:GIOIVB>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT DIABETES-MELLITUS; POSTMENOPAUSAL WOMEN; GLYCEMIC CONTROL; RISK-FACTORS; IN-VITRO; INSULIN; FRACTURE; DENSITY; CALCIUM; INVITRO;
Keywords:
glucose; osteoblast; calcium; bone nodule; mineralization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Sprague, SM Northwestern Univ, Div Nephrol, Evanston NW Healthcare, Sch Med,Dept Med, 2650 Ridge Ave, Evanston, IL 60201 USA Northwestern Univ 2650 Ridge Ave Evanston IL USA 60201 201 USA
Citazione:
E. Balint et al., "Glucose-induced inhibition of in vitro bone mineralization", BONE, 28(1), 2001, pp. 21-28

Abstract

Patients with diabetes tend to have an increased incidence of osteopenia that may be related to hyperglycemia. However, little is known about how glucose may alter bone formation and osteoblast maturation. To determine whether glucose affects osteoblastic calcium deposition, MC3T3-E1 cells were incubated in media containing either a normal (5.5 mmol/L) or high glucose concentration (15 mmol/L) or mannitol (15 mmol/L), and bone nodule formation was examined. Net calcium flux was measured thrice weekly and cumulative calcium uptake was determined, Compared with control incubations, glucose significantly inhibited daily and cumulative calcium uptake into the nodules, At the time of matrix maturation, cultures undergo a rapid phase of increased calcium deposition; this was significantly inhibited by the presence of glucose, Total calcium uptake, determined by acid digestion, was also significantly inhibited by glucose. Area and number of nodules were quantitated at the end of the incubation period (day 30) by staining with Alizarin Red Scalcium stain. Compared with both control and mannitol-treated cultures, the number of nodules was increased by incubation with glucose. Furthermore,both the average total nodular area and calcified nodular area of large nodules were increased by glucose, Cellular proliferation as well as the release of markers of osteoblast activity (osteocalcin and alkaline phosphatase) were determined at the end of the experimental period (day 30), Cellular proliferation and alkaline phosphatase activity was significantly increasedin the presence of glucose, however, the release of osteocalcin into culture media was similar in all three groups. In conclusion, the present study shows that elevated glucose concentration present throughout the development of murine osteoblasts stimulates cellular proliferation while inhibiting calcium uptake. The result of glucose inhibition of calcium uptake suggeststhat bone could be structurally altered in diabetes. (C) 2001 by Elsevier Science Inc. All rights reserved.

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Documento generato il 27/01/20 alle ore 01:43:27