Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A single mutation in the NAD-specific formate dehydrogenase from Candida methylica allows the enzyme to use NADP
Autore:
Gul-Karaguler, N; Sessions, RB; Clarke, AR; Holbrook, JJ;
Indirizzi:
Univ Bristol, Sch Med Sci, Dept Biochem, Bristol BS8 1TD, Avon, England Univ Bristol Bristol Avon England BS8 1TD Bristol BS8 1TD, Avon, England
Titolo Testata:
BIOTECHNOLOGY LETTERS
fascicolo: 4, volume: 23, anno: 2001,
pagine: 283 - 287
SICI:
0141-5492(200102)23:4<283:ASMITN>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATALYZED ORGANIC-SYNTHESIS; LACTATE-DEHYDROGENASE; REGENERATION; COENZYME;
Keywords:
coenzyme specificity; dehydrogenase; formate; kinetics; modelling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Sessions, RB Univ Bristol, Sch Med Sci, Dept Biochem, Univ Walk, Bristol BS8 1TD, Avon,England Univ Bristol Univ Walk Bristol Avon England BS8 1TD n,England
Citazione:
N. Gul-Karaguler et al., "A single mutation in the NAD-specific formate dehydrogenase from Candida methylica allows the enzyme to use NADP", BIOTECH LET, 23(4), 2001, pp. 283-287

Abstract

An homology model of Candida methylica formate dehydrogenase (cmFDH) was constructed based on the Pseudomonas sp. 101 formate dehydrogenase (psFDH) structure. An aspartic acid residue in the model was predicted to interact with the adenine ribose of the NAD cofactor, in common with many NAD-dependent oxoreductases. Replacement of this aspartic acid residue by serine in cmFDH removed the absolute requirement for NAD over NADP shown by the wild type enzyme. Taken with similar results shown by d- and l-lactate dehydrogenases, this suggests that an aspartic acid in this position is a major determinant of coenzyme specificity in NAD/NADP-dependent dehydrogenases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 19:53:53