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Titolo:
Comparison of photovoltaic behaviors for horseradish peroxidase and its mimicry by surface photovoltage spectroscopy
Autore:
Wang, XQ; Li, LS; Van der Meer, BW; Jin, J; Tang, DX; Hui, Z; Li, YZ; Li, TJ;
Indirizzi:
Western Kentucky Univ, Dept Phys & Astron, Bowling Green, KY 42101 USA Western Kentucky Univ Bowling Green KY USA 42101 ling Green, KY 42101 USA Peking Univ, Dept Chem, Beijing 100871, Peoples R China Peking Univ Beijing Peoples R China 100871 ijing 100871, Peoples R China Jilin Univ, Dept Chem, Ctr Intelligent Mat United Res, Changchun 130023, Peoples R China Jilin Univ Changchun Peoples R China 130023 chun 130023, Peoples R China Univ Louisville, Dept Ophthalmol & Visual Sci, Louisville, KY 40292 USA Univ Louisville Louisville KY USA 40292 ual Sci, Louisville, KY 40292 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
fascicolo: 1-2, volume: 1544, anno: 2001,
pagine: 333 - 340
SICI:
0167-4838(20010112)1544:1-2<333:COPBFH>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDASE REACTION; P-PHENYLPHENOL; BIOSENSOR; HEMIN; IMMOBILIZATION; OSCILLATIONS; COMPLEXES; MICELLES; BINDING; CYANIDE;
Keywords:
hemin; peroxidase mimesis; proximal ligand; heterostructure; interface; light-induced electron transfer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Van der Meer, BW Western Kentucky Univ, Dept Phys & Astron, Bowling Green,KY 42101 USA Western Kentucky Univ Bowling Green KY USA 42101 101 USA
Citazione:
X.Q. Wang et al., "Comparison of photovoltaic behaviors for horseradish peroxidase and its mimicry by surface photovoltage spectroscopy", BBA-PROT ST, 1544(1-2), 2001, pp. 333-340

Abstract

Surface photovoltage spectroscopy (SPS) was chosen to study the photovoltaic behavior of horseradish peroxidase (HRP), hemin and immobilized hemin (poly(NIPAAm/MBA/hemin)). Different photovoltaic behaviors were observed in these three systems. In air, similar SPS curves were found for HRP and poly(NIPAAm/MEIA/hemin) with different response intensities. However, poly(NIPAAm/MBA/hemin) showed a wider changing range upon increasing the positive andnegative bias to 1.0 V. The SPS of hemin showed a total different behaviorwhen an external positive potential was applied. In vacuum, clearly different photovoltaic behaviors were found. Moreover, the response value decreased when HRP was exposed to O-2, the SPS intensity was different from that in air, and could be altered by changing the external biases. On the other hand, the SPS could not be changed before and after poly(NIPAAm/MBA/hemin) was exposed to Oz. These differences may result from different chemical microenvironments for hemin in HRP versus that in poly(NIPAAm/MBAlhemin). It could be concluded that H2O and O-2 were important factors affecting the photovoltage response in HRP, but only H2O played this important role in poly(NIPAAM/MBA/hemin). (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 09/07/20 alle ore 18:37:58