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Titolo:
VEGF can act as vascular permeability factor in the hepatic sinusoids through upregulation of porosity of endothelial cells
Autore:
Funyu, J; Mochida, S; Inao, M; Matsui, A; Fujiwara, K;
Indirizzi:
Saitama Med Sch, Dept Internal Med 3, Moroyama, Saitama 3500495, Japan Saitama Med Sch Moroyama Saitama Japan 3500495 ma, Saitama 3500495, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 280, anno: 2001,
pagine: 481 - 485
SICI:
0006-291X(20010119)280:2<481:VCAAVP>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASE RECEPTOR FAMILY; GROWTH-FACTOR; TYROSINE KINASE; RAT-LIVER; FLT-1; ANGIOGENESIS; EXPRESSIONS; KDR/FLK-1; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Funyu, J Saitama Med Sch, Dept Internal Med 3, 38 Morohongo, Moroyama, Saitama 3500495, Japan Saitama Med Sch 38 Morohongo Moroyama Saitama Japan 3500495 Japan
Citazione:
J. Funyu et al., "VEGF can act as vascular permeability factor in the hepatic sinusoids through upregulation of porosity of endothelial cells", BIOC BIOP R, 280(2), 2001, pp. 481-485

Abstract

VEGF is shown to be a vascular permeability factor (VPF) as well as a growth stimulatory factor on endothelial cells. In the hepatic sinusoids, endothelial cells express flt-1 and KDR/flk-1, receptors for VEGF. These cells, in primary culture, proliferate in response to VEGF stimulation. However, the role of VEGF as VPF in the hepatic sinusoids is to be elucidated. The effect of VEGF on the porosity of sinusoidal endothelial cells was studied. Sinusoidal endothelial cells were isolated from rats and cultured in DMEM containing 10% FCS on plastic dishes coated with type I collagen for 16 and 48 h for morphological examination and cell-number measurement, respectively. When the cells were cultured without VEGFF addition, their number was decreased at 48 h compared to that at 16 h. However, the number was unchanged in the cells cultured with VEGF at 10 ng/mL and increased with addition of VEGF at 100 ng/mL. Scanning electron microscopic examination revealed that sieve-plate appearance of the cells was impaired in culture with no VEGF addition, but the appearance was maintained in culture with VEGF at 10 ng/mL or more. The cells cultured with VEGF at 100 ng/mL showed significantly increased number and size of pores compared to the cells cultured with VEGF at10 ng/mL, suggesting that sinusoidal endothelial cells proliferating in response to VEGF may increase their porosity. It is concluded that VEGF can act as VPF in the hepatic sinusoids through regulation of endothelial cell porosity. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 06:06:59