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Titolo:
Bothrops moojeni venom kills Leishmania spp. with hydrogen peroxide generated by its L-amino acid oxidase
Autore:
Tempone, AG; Andrade, HF; Spencer, PJ; Lourenco, CO; Rogero, JR; Nascimento, N;
Indirizzi:
CNEN SP, IPEN, Div Radiobiol, BR-05508900 Sao Paulo, Brazil CNEN SP Sao Paulo Brazil BR-05508900 BCol, BR-05508900 Sao Paulo, Brazil FMUSP, Inst Med Trop Sao Paulo, Protozool Lab, BR-05403000 Sao Paulo, Brazil FMUSP Sao Paulo Brazil BR-05403000 BC Lab, BR-05403000 Sao Paulo, Brazil
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 280, anno: 2001,
pagine: 620 - 624
SICI:
0006-291X(20010126)280:3<620:BMVKLS>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
COBRA OPHIOPHAGUS-HANNAH; DIFFERENT SNAKE-VENOMS; PHARMACOLOGICAL ACTION; PSEUDECHIS-AUSTRALIS; PURIFICATION; IDENTIFICATION; INHIBITION; APOPTOSIS; INFANTUM; PROTEINS;
Keywords:
Leishmaniasis; parasites; Bothrops moojeni; snake venom; L-amino acid oxidase; Leishmania amazonensis; Leishmania panamensis; Leishmania chagasi;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Tempone, AG CNEN SP, IPEN, Div Radiobiol, Travessa R 400,Cid Univ, BR-05508900 Sao Paulo, Brazil CNEN SP Travessa R 400,Cid Univ Sao Paulo Brazil BR-05508900 BC
Citazione:
A.G. Tempone et al., "Bothrops moojeni venom kills Leishmania spp. with hydrogen peroxide generated by its L-amino acid oxidase", BIOC BIOP R, 280(3), 2001, pp. 620-624

Abstract

Leishmaniasis is an endemic tropical disease in South America, with few therapeutic approaches. Snake venoms are complex protein mixtures with biological actions that could be used as tools for drug development. Here we showthat Bothrops moojeni crude venom presented a killing effect in vitro against Leishmania spp. promastigotes, but not with amastigotes, as determined by a viability assay using the mitochondrial oxidative function. Purification of active fractions from crude venom was performed by molecular exclusion and ion exchange chromatography. Anti-Leishmania and L-amino acid oxidase(L-AAO, EC.1.4.3.2.) activities co-eluted in the same fractions. The molecular weight of the active enzyme was estimated to be 140 kDa by molecular exclusion chromatography, and 69 kDa by SDS-PAGE, with a 4.8 isoelectric point. Using substrate subtraction and catalase for scavenging, the action of L-AAO was demonstrated to be hydrogen-peroxide-dependent. (C) 2001 AcademicPress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 10:13:23