Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Free fatty acid effects on mitochondrial permeability: An overview
Autore:
Sultan, A; Sokolove, PM;
Indirizzi:
Univ Maryland, Sch Med, Dept Pharmacol & Expt Therapeut, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 t Therapeut, Baltimore, MD 21201 USA
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 1, volume: 386, anno: 2001,
pagine: 52 - 61
SICI:
0003-9861(20010201)386:1<52:FFAEOM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-LIVER MITOCHONDRIA; MULTIPLE CONDUCTANCE CHANNEL; CYTOCHROME-C-OXIDASE; TRANSITION PORE; CYCLOSPORINE-A; INORGANIC-PHOSPHATE; PHASE-TRANSITION; INNER MEMBRANE; PALMITIC ACID; HIGH-AFFINITY;
Keywords:
rat liver mitochondria; permeability transition; cyclosporin A; free fatty acids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Sokolove, PM Grad Sch, 621 W Lombard St,Suite 201, Baltimore, MD 21201 USAGrad Sch 621 W Lombard St,Suite 201 Baltimore MD USA 21201 SA
Citazione:
A. Sultan e P.M. Sokolove, "Free fatty acid effects on mitochondrial permeability: An overview", ARCH BIOCH, 386(1), 2001, pp. 52-61

Abstract

A variety of experimental conditions elicit increases in mitochondrial permeability that can be differentiated from the classic cyclosporin A (CsA)-sensitive mitochondrial permeability transition (MPT). For example, butylated hydroxytoluene, signal peptides, and the hormone thyroxine have been shown to promote increases in mitochondrial permeability that are CsA-insensitive. Our laboratory has recently demonstrated that palmitic acid, a saturated Is-carbon free fatty acid (FFA), can also open a CsA-insensitive pore. This nonclassic permeability transition (NCPT) is further distinguished by a nonselective dependence on divalent cations and by spontaneous closure. To determine if induction of the NCPT is specific to palmitic acid and to resolve conflicting reports as to the mechanisms by which FFAs alter mitochondrial permeability, we examined in detail mitochondrial swelling induced by FFAs that differ in chain length and degree of saturation. The following results were obtained: (1) In the presence of modest Ca2+ concentrations (75 nmol/mg protein), medium-chain FFAs (C12-C18) were more effective in eliciting mitochondrial swelling than were shorter or longer FFAs; medium-chain alkanols and amines had no effect. (2) Under these conditions, saturated FFAsinduced CsA-insensitive swelling with all the characteristics of the NCPT,while unsaturated FFAs triggered the MPT, (3) When matrix Ca2+ concentration was further elevated, unsaturated FFAs, triggered the NCPT. (4) Mitochondrial swelling induced by saturated FFAs was inhibited by unsaturated FFAs but not by other saturated FFAs or medium-chain alkanols, These results suggest that ambient conditions can greatly influence the nature of the increase in mitochondrial permeability induced by FFAs, They are also consistent with our earlier proposal that Ca2+ (Or Sr2+) binding to FFAs in the inner leaflet of the inner mitochondrial membrane underlies the NCPT. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 07:01:43