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Titolo:
Controlled cell deformation produces defined areas of contact between cells and ligand-coated surfaces
Autore:
Patrick, SM; Kim, S; Braunstein, NS; Maldarelli, CM; Thomas, JL; Leonard, EF;
Indirizzi:
Columbia Univ, Dept Chem Engn, New York, NY 10027 USA Columbia Univ New York NY USA 10027 ept Chem Engn, New York, NY 10027 USA Columbia Univ, Dept Med, New York, NY 10027 USA Columbia Univ New York NYUSA 10027 niv, Dept Med, New York, NY 10027 USA CUNY City Coll, Dept Chem Engn, New York, NY 10031 USA CUNY City Coll NewYork NY USA 10031 pt Chem Engn, New York, NY 10031 USA
Titolo Testata:
ANNALS OF BIOMEDICAL ENGINEERING
fascicolo: 1, volume: 29, anno: 2001,
pagine: 1 - 8
SICI:
0090-6964(200101)29:1<1:CCDPDA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATION; ADHESION; KINETICS;
Keywords:
contact area; biophysical forces; receptor-ligand interaction; cell deformation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Leonard, EF Columbia Univ, Dept Chem Engn, Mudd Bldg,Rm 814,500 W 120th St, New York, NY 10027 USA Columbia Univ Mudd Bldg,Rm 814,500 W 120th St New York NY USA 10027
Citazione:
S.M. Patrick et al., "Controlled cell deformation produces defined areas of contact between cells and ligand-coated surfaces", ANN BIOMED, 29(1), 2001, pp. 1-8

Abstract

A method which allows precise control of the time of initiation and the area of contact of T cells with immobilized ligands has been developed. Cellsare trapped in an asymmetric film that can be quantitatively thinned by reducing the film's capillary pressure. Ligands adsorbed to the base of the apparatus are forced into close contact with the cells as the air-liquid interface is drawn down. Using interference microscopy and microbeads to indicate the film height, the amount of thinning can be controlled to within 1 mum. In this study, this system was used to produce contact areas of 182 and356 mum(2) between T cells and anti-CD3 coated surfaces. These contact areas were measured using fluorescent dye exclusion microscopy. This apparatuscan be used for quantitative studies of T cell activation, as is reported in Patrick ct al., J. Immunol. Method. 24:97-108, 2000. (C) 2001 BiomedicalEngineering Society.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:35:53