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Titolo:
Absence of direct antioxidant effects from volatile anesthetics in primarymixed neuronal-glial cultures
Autore:
Kudo, M; Aono, M; Lee, Y; Massey, G; Pearlstein, RD; Warner, DS;
Indirizzi:
Duke Univ, Med Ctr, Dept Anesthesiol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Ctr, Dept Anesthesiol, Durham, NC 27710 USA Duke Univ, Med Ctr, Dept Surg, Multidisciplinary Neuroprotect Res Labs, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Neuroprotect Res Labs, Durham, NC 27710 USA
Titolo Testata:
ANESTHESIOLOGY
fascicolo: 2, volume: 94, anno: 2001,
pagine: 303 - 312
SICI:
0003-3022(200102)94:2<303:AODAEF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
FREE-RADICAL FORMATION; PARTITION-COEFFICIENTS; LIPID-PEROXIDATION; CEREBRAL-ISCHEMIA; BRAIN TEMPERATURE; OXIDATIVE STRESS; GLOBAL-ISCHEMIA; CELL-DEATH; HALOTHANE; ISOFLURANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Warner, DS Duke Univ, Med Ctr, Dept Anesthesiol, Box 3094, Durham, NC 27710 USA Duke Univ Box 3094 Durham NC USA 27710 94, Durham, NC 27710 USA
Citazione:
M. Kudo et al., "Absence of direct antioxidant effects from volatile anesthetics in primarymixed neuronal-glial cultures", ANESTHESIOL, 94(2), 2001, pp. 303-312

Abstract

Background: Volatile anesthetics decrease ischemic brain injury. Mechanisms for this protection remain under investigation. The authors hypothesized that volatile anesthetics serve as antioxidants in a neuronal-glial cell culture system,Methods: Primary cortical neuronal-glial cultures were prepared from fetalrat brain. Cultures were exposed to iron, H2O2, or xanthine-xanthine oxidase for 30 min in serum-free media containing dissolved isoflurane (0-3.2 mr), sevoflurane (0-3.6 mr), halothane (0-4.1 mu), n-hexanol, or known antioxidants, Cell damage was assessed by release of lactate dehydrogenase (LDH) and trypan blue exclusion 24 h later. Lipid peroxidation was measured by the production of thiobarbituric acid-reactive substances in a cell-free lipid system. Iron and calcium uptake and mitochondrial depolarization were measured after exposure to iron in the presence or absence of isoflurane. Results: Deferoxamine reduced LDH release caused by H2O2, or xanthine-xanthine oxidase, but the volatile anesthetics had no effect. Iron-induced LDH release was prevented by the volatile anesthetics (maximum effect for halothane = 1.2 mM, isoflurane = 1.2 mM, and sevoflurane = 2.1 mM aqueous phase). When corrected for lipid solubility, the three volatile anesthetics were equipotent against iron-induced LDH release. In the cell-free system, therewas no effect of the anesthetics on thiobarbituric acid-reactive substanceformation in contrast to Trolox, which provided complete inhibition. Isoflurane (1.2 mM) reduced mean iron uptake by 46% and inhibited mitochondrial depolarization but had no effect on calcium uptake. Conclusions: Volatile anesthetics reduced cell death induced by oxidative stress only in the context of iron challenge. The likely reason for protection against iron toxicity is inhibition of iron uptake and therefore indirect reduction of subsequent intracellular oxidative stress caused by this challenge. These data argue against a primary antioxidant effect of volatile anesthetics.

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Documento generato il 09/04/20 alle ore 10:36:50