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Titolo:
Involvement of mu-calpain in human sperm capacitation for fertilization
Autore:
Aoyama, T; Ozaki, Y; Aoki, K; Kunimatsu, M; Tada, T; Sasaki, M; Suzumori, K;
Indirizzi:
Nagoya City Univ, Sch Med, Dept Obstet & Gynecol, Mizuho Ku, Nagoya, Aichi4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 , Nagoya, Aichi4678601, Japan Nagoya City Univ, Sch Med, Dept Biochem, Mizuho Ku, Nagoya, Aichi 4678601,Japan Nagoya City Univ Nagoya Aichi Japan 4678601 , Nagoya, Aichi 4678601,Japan Nagoya City Johsai Hosp, Dept Obstet & Gynecol, Nakamura Ku, Nagoya, Aichi4530815, Japan Nagoya City Johsai Hosp Nagoya Aichi Japan 4530815 a, Aichi4530815, Japan Nagoya City Univ, Sch Med, Dept Pathol, Mizuho Ku, Nagoya, Aichi 4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Nagoya, Aichi 4678601, Japan
Titolo Testata:
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
fascicolo: 1, volume: 45, anno: 2001,
pagine: 12 - 20
SICI:
1046-7408(200101)45:1<12:IOMIHS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED NEUTRAL PROTEASE; ACROSOME REACTION; INTRACELLULAR CALCIUM; HAMSTER SPERMATOZOA; LARGE SUBUNIT; PROGESTERONE; PROTEOLYSIS; MOTILITY; REPERFUSION; INHIBITORS;
Keywords:
acrosome reaction; calpain; calpain inhibitor; human sperm; sperm motility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Ozaki, Y Nagoya City Univ, Sch Med, Dept Obstet & Gynecol, Mizuho Ku, Nagoya, Aichi4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Aichi4678601, Japan
Citazione:
T. Aoyama et al., "Involvement of mu-calpain in human sperm capacitation for fertilization", AM J REPROD, 45(1), 2001, pp. 12-20

Abstract

PROBLEM: The distribution and physiological role of calpains in human sperm were investigated. METHOD OF STUDY: Semen collected manually from healthy donors was liquefied then centrifuged by percoll gradient centrifugation. After exposure to different concentrations of Ca2+ ionophore A(23187), the samples were used for immunostaining sodium dodecyl sulfate polyacrylamide gel electrophoresis,and western blot analysis. It was speculated from the results of the studyusing calpain-specific inhibitors that calpain contributes to the sperm motility and acrosome reaction. RESULTS: With the anti-pro mu -calpain antibody, sperm were immunostained,whereas all were negative for anti-pro m-calpain antibody binding. Stainedsperm were classified into four types according to the staining pattern: acrosome type, equatorial segment type, whole head type, and neck and tail segments type. Western blot analysis of sperm homogenate revealed a single 80-kDa band using the anti-pro mu -calpain antibody, its dose-dependent reduction with Ca2+ ionophore A(23187) suggesting activation by this treatment. In the presence of membrane permeable calpain inhibitors, sperm motility and acrosome reaction were significantly suppressed. CONCLUSION: These results indicate that mu -calpain may play pivotal rolesin the process of fertilization of human sperm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:49:39