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Titolo:
The production and clinical evaluation of macrophage colony-stimulating factor and macrophage chemoattractant protein-1 in human follicular fluids
Autore:
Kawano, Y; Kawasaki, F; Nakamura, S; Matsui, N; Narahara, H; Miyakawa, I;
Indirizzi:
Oita Med Univ, Dept Obstet & Gynecol, Oita, Japan Oita Med Univ Oita Japan a Med Univ, Dept Obstet & Gynecol, Oita, Japan
Titolo Testata:
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
fascicolo: 1, volume: 45, anno: 2001,
pagine: 1 - 5
SICI:
1046-7408(200101)45:1<1:TPACEO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO FERTILIZATION; HUMAN OOCYTES INVITRO; LEUKOCYTE SUBSETS; GRANULOSA-CELL; RAT OVARY; OVULATION; GONADOTROPIN; PURIFICATION; LOCALIZATION; PROGESTERONE;
Keywords:
follicular fluids; IVF-ET; macrophage chemoattractant protein-1; macrophage colony-stimulating factor; oocyte maturation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Kawano, Y Oita Med Univ, Dept Obstet & Gynecol, Oita, Japan Oita Med UnivOita Japan , Dept Obstet & Gynecol, Oita, Japan
Citazione:
Y. Kawano et al., "The production and clinical evaluation of macrophage colony-stimulating factor and macrophage chemoattractant protein-1 in human follicular fluids", AM J REPROD, 45(1), 2001, pp. 1-5

Abstract

PROBLEM: In order to investigate the role of macrophage colony-stimulatingfactor (M-CSF) and macrophage chemoattractant protein-1 (MCP-1) in human ovulation, we measured the concentrations of M-CSF and MCP-1 in human follicular fluids (FFs) and correlated them with oocyte maturation. METHOD OF STUDY: The oocytes were obtained from the FFs of 46 women undergoing in vitro fertilization and embryo transfer (IVF-ET). The concentrations of M-CSF and MCP-1 in the FFs were measured by enzyme-linked immunosorbent assay. In addition, granulosa cells obtained from the FFs of IVF patientswere cultured and treated with forskolin and 12-O-tetradecanoylphorbol 13-acetate (TPA) for 24-48 hr. RESULTS: Concentrations of M-CSF and MCP-1 were significantly higher in the FFs than in the serum (P < 0.01). M-CSF concentrations tended to be higher, while MCP-1 concentrations were significantly higher in the FFs containing mature oocytes than in FFs containing immature oocytes (P < 0.05). The production of M-CSF was markedly increased over the basal level after treatment with forskolin (10 muM) for 24 (P < 0.02) and 48 hr (P < 0.01); however, the production of MCP-1 was unchanged. CONCLUSIONS: Our data suggest that M-CSF and MCP-1 may play an important role in human preovulatory processes and that M-CSF, in particular, may be regulated by cyclic adenosine monophosphate. M-CSF and MCP-1 may also be valuable biochemical markers in the evaluation of oocyte maturation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:57:34