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Titolo:
NO donor sodium nitroprusside inhibits excitation-contraction coupling in guinea pig taenia coli
Autore:
Kwon, SC; Ozaki, H; Karaki, H;
Indirizzi:
Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Pharmacol, Bunkyo Ku, Tokyo 1138657, Japan Univ Tokyo Tokyo Japan 1138657 harmacol, Bunkyo Ku, Tokyo 1138657, Japan Yonsei Univ, Coll Med, Dept Physiol, Seoul 120752, South Korea Yonsei Univ Seoul South Korea 120752 Physiol, Seoul 120752, South Korea
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 6, volume: 279, anno: 2000,
pagine: G1235 - G1241
SICI:
0193-1857(200012)279:6<G1235:NDSNIE>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; NONSELECTIVE CATION CHANNELS; DEPENDENT PROTEIN-KINASE; NITRIC-OXIDE; MEDIATED RELAXATIONS; CALCIUM MOVEMENTS; DIVALENT-CATIONS; CA2+ CURRENTS; K-CHANNELS; ILEUM;
Keywords:
intracellular calcium concentration; calcium channel; potassium channel; nonselective cation channel; myosin light chain phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Ozaki, H Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Pharmacol, Bunkyo Ku, Yayoi 1-1-1, Tokyo 1138657, Japan Univ Tokyo Yayoi 1-1-1 Tokyo Japan 1138657 Tokyo 1138657, Japan
Citazione:
S.C. Kwon et al., "NO donor sodium nitroprusside inhibits excitation-contraction coupling in guinea pig taenia coli", AM J P-GAST, 279(6), 2000, pp. G1235-G1241

Abstract

In guinea pig taenia coli, the nitric oxide (NO) donor sodium nitroprusside (SNP, 1 muM) reduced the carbachol-stimulated increases in muscle force in parallel with a decrease in intracellular Ca2+ concentration ([Ca2+](i)). A decrease in the myosin light chain phosphorylation was also observed that was closely correlated with the decrease in [Ca2+](i). With the patch-clamp technique, 10 muM SNP decreased the peak Ba2+ current, and this effect was blocked by an inhibitor of soluble guanylate cyclase. Carbachol (10 muM)induced an inward current, and this effect was markedly inhibited by SNP. SNP markedly increased the depolarization-activated outward K+ currents, and this current was completely blocked by 0.3 muM iberiotoxin. SNP (1 muM) significantly increased cGMP content without changing cAMP content. Decreased Ca2+ sensitivity by SNP of contractile elements was not prominent in the permeabilized taenia, which was consistent with the [Ca2+](i)-force relationship in the intact tissue. These results suggest that SNP inhibits myosin light chain phosphorylation and smooth muscle contraction stimulated by carbachol, mainly by decreasing [Ca2+](i), which resulted from the combinationof the inhibition of voltage-dependent Ca2+ channels, the inhibition of nonselective cation currents, and the activation of Ca2+-activated K+ currents.

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Documento generato il 11/07/20 alle ore 16:59:29