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Titolo:
Gene transfer systems derived from visna virus: Analysis of virus production and infectivity
Autore:
Berkowitz, RD; Ilves, H; Plavec, I; Veres, G;
Indirizzi:
SyStemix Inc, Palo Alto, CA 94304 USA SyStemix Inc Palo Alto CA USA 94304SyStemix Inc, Palo Alto, CA 94304 USA
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 279, anno: 2001,
pagine: 116 - 129
SICI:
0042-6822(20010105)279:1<116:GTSDFV>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONDIVIDING HUMAN-CELLS; LENTIVIRAL VECTOR; NUCLEOTIDE-SEQUENCE; RETROVIRAL VECTORS; HIGHLY EFFICIENT; HIGH-TITER; EXPRESSION; TRANSDUCTION; REPLICATION;
Keywords:
visna; lentivirus; vector; gene therapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Berkowitz, RD 32 Dorland St,2, San Francisco, CA 94110 USA 32 Dorland St,2 San Francisco CA USA 94110 co, CA 94110 USA
Citazione:
R.D. Berkowitz et al., "Gene transfer systems derived from visna virus: Analysis of virus production and infectivity", VIROLOGY, 279(1), 2001, pp. 116-129

Abstract

Efficient transfer of therapeutic genes into nondividing human cells can be accomplished by inserting the genes into lentiviruses and infecting the cells with the modified viruses. The most developed lentivirus gene transfersystems are based on HIV-1, but because of the widespread HIV epidemic, the use of HIV-based vectors for gene therapy may be associated with a safetyrisk. In an attempt to find another lentivirus which can transduce human cells and might be safer than HIV-1, we generated gene transfer constructs based on the sheep lentivirus Visna. Molecular analysis of the constructs ina transient production system indicated that Visna produced as many maturevirus particles as did HIV-1. Moreover, the virus particles incorporated aheterologous surface protein marker-gene-containing vector RNAs as efficiently as did HIV-1. However, the visna virus transduced target cells poorly because of defects in reverse transcription and integration of the vector. Further modifications must be made to the Visna gene transfer system if thesystem is to be used in clinical gene therapy applications. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 05:30:37