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Titolo:
Genetic and biological variation in equine infectious anemia virus rev correlates with variable stages of clinical disease in an experimentally infected pony
Autore:
Belshan, M; Baccam, P; Oaks, JL; Sponseller, BA; Murphy, SC; Cornette, J; Carpenter, S;
Indirizzi:
Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA IowaState Univ Ames IA USA 50011 obiol & Prevent Med, Ames, IA 50011 USA Iowa State Univ, Dept Math, Ames, IA 50011 USA Iowa State Univ Ames IA USA 50011 ate Univ, Dept Math, Ames, IA 50011 USA Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 Pathol, Pullman, WA 99164 USA
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 279, anno: 2001,
pagine: 185 - 200
SICI:
0042-6822(20010105)279:1<185:GABVIE>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS; REVERSE-TRANSCRIPTASE; HIV-1 REV; HYPERVARIABLE REGION; PERSISTENT INFECTION; REGULATORY PROTEINS; ACTIVATION DOMAIN; IMMUNE-RESPONSES; RNA; PCR;
Keywords:
EIAV; Rev; persistence; quasispecies; variation; evolution;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Carpenter, S Iowa State Univ, Dept Vet Microbiol & Prevent Med, Room 1136,Vet Med,S 16th St, Ames, IA 50011 USA Iowa State Univ Room 1136,Vet Med,S 16th St Ames IA USA 50011
Citazione:
M. Belshan et al., "Genetic and biological variation in equine infectious anemia virus rev correlates with variable stages of clinical disease in an experimentally infected pony", VIROLOGY, 279(1), 2001, pp. 185-200

Abstract

Genetic and biological variation in the regulatory protein Rev of equine infectious anemia virus (EIAV) were examined throughout a clinically dynamicdisease course of an experimentally infected pony. Following infection with the virulent EIAV(Wyo), the pony underwent a Variable disease course, including an acute fever episode at 12 days postinfection (DPI), multiple recurrent fever episodes until 135 DPI, a prolonged subclinical period, and twolate fever episodes. Viral RNA was isolated from the inoculum and sequential sera samples, and the rev exon 2/gp45 overlapping ORFs were amplified, cloned, and sequenced. Novel variants were found throughout infection, and genetic analyses indicated that both the Rev and gp45 ORFs were under selective pressure. The Rev variant predominant in the inoculum, R1, remained predominant during the early periods following infection (until 35 DPI); however, R1 was replaced by new predominant variants during the recurrent fever period (67-135 DPI). R1 reemerged as the predominant variant during the afebrile period, but a new predominant variant, R93, was associated with the late fever episodes. Rev Variants predominant during recurrent febrile and late-febrile periods had significantly higher Rev-mediated nuclear export activity than the variants predominant during the acute and afebrile periods. Statistical correlation was found between Rev activity and different stages of clinical disease. Together, these results suggest that genetic and biological variation in rev may be a contributing factor in EIAV disease progression. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 13:27:26