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Titolo:
The toxic responses induced by okadaic acid involve processing of multiplecaspase isoforms
Autore:
Rossini, GP; Sgarbi, N; Malaguti, C;
Indirizzi:
Univ Modena, Sez Chim Biol, Dipartimento Sci Biomed, I-41100 Modena, ItalyUniv Modena Modena Italy I-41100 mento Sci Biomed, I-41100 Modena, Italy
Titolo Testata:
TOXICON
fascicolo: 6, volume: 39, anno: 2001,
pagine: 763 - 770
SICI:
0041-0101(200106)39:6<763:TTRIBO>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-INDUCED APOPTOSIS; BREAST-CARCINOMA CELLS; PROTEIN PHOSPHATASES; NECROSIS-FACTOR; ICE/CED-3 PROTEASE; DEATH; ACTIVATION; INHIBITORS; INDUCTION; KINASE;
Keywords:
okadaic acid; protein phoshatase; apoptosis; cytotoxicity; phycotoxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Rossini, GP Univ Modena, Sez Chim Biol, Dipartimento Sci Biomed, Via Campi287, I-41100 Modena, Italy Univ Modena Via Campi 287 Modena Italy I-41100 Modena, Italy
Citazione:
G.P. Rossini et al., "The toxic responses induced by okadaic acid involve processing of multiplecaspase isoforms", TOXICON, 39(6), 2001, pp. 763-770

Abstract

The recognized role of caspases as executioners of apoptosis, led us to investigate their involvement in death responses induced by okadaic acid (OA)in HeLa S-3 and MCF-7 cells. A one-day treatment with OA induced accumulation of the 85 kDa poly(ADP-ribose) polymerase (PARP) fragment in cell lysates but the response was prevented if cells were treated with OA in the presence of the caspase inhibitors Z-VAD-FMK and Z-DEVD-FMK. The HeLa S-3 and MCF-7 cells were found to contain measurable levels of the intact caspase-2,-7, -g and -9 zymogens, whereas caspase-3 was found only in HeLa cells. After one day of OA treatment, pro-caspase-2, -3, -7 and -9 isoforms were found processed in HeLa cells, whereas only pro-caspase-2 was processed in MCF-7 cells. Pro-caspase-8, in turn, was mostly unprocessed in both cell lines. The possible interference of caspase inhibitors on cell death was also evaluated, and we found that both Z-VAD-FMK and Z-DEVD-FMK could contribute different extents of protection of MCF-7 and HeLa cells from toxic effects caused by OA. We concluded that OA triggers multiple pathways of caspase processing, contributing to death responses triggered by OA in HeLa S3 and MCF-7 cells. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:47:21