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Titolo:
Detection of dichromate (VI)-induced DNA strand breaks and formation of paramagnetic chromium in multiple mouse organs
Autore:
Ueno, S; Kashimoto, T; Susa, N; Furukawa, Y; Ishii, M; Yokoi, K; Yasuno, M; Sasaki, YF; Ueda, J; Nishimura, Y; Sugiyama, M;
Indirizzi:
Kitasato Univ, Sch Vet Med & Anim Sci, Lab Vet Publ Hlth, Towada, Aomori 0348628, Japan Kitasato Univ Towada Aomori Japan 0348628 , Towada, Aomori 0348628, Japan Hachinohe Natl Coll Technol, Lab Genotox, Hachinohe, Aomori 0391192, JapanHachinohe Natl Coll Technol Hachinohe Aomori Japan 0391192 0391192, Japan Natl Inst Radiol Sci, Inage Ku, Chiba 2638555, Japan Natl Inst Radiol SciChiba Japan 2638555 Inage Ku, Chiba 2638555, Japan Sugiyama Pharm, Yamada, Fukuoka 8210011, Japan Sugiyama Pharm Yamada Fukuoka Japan 8210011 amada, Fukuoka 8210011, Japan
Titolo Testata:
TOXICOLOGY AND APPLIED PHARMACOLOGY
fascicolo: 1, volume: 170, anno: 2001,
pagine: 56 - 62
SICI:
0041-008X(20010101)170:1<56:DOD(DS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HAMSTER V-79 CELLS; ALKALI-LABILE SITES; SODIUM CHROMATE(VI); GLUTATHIONE-REDUCTASE; HYDROGEN-PEROXIDE; RADICAL FORMATION; DG HYDROXYLATION; DAMAGE; CYTOTOXICITY; LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ueno, S Kitasato Univ, Sch Vet Med & Anim Sci, Lab Vet Publ Hlth, Higashi 23-35-1,Towada, Aomori 0348628, Japan Kitasato Univ Higashi 23-35-1 Towada Aomori Japan 0348628 8, Japan
Citazione:
S. Ueno et al., "Detection of dichromate (VI)-induced DNA strand breaks and formation of paramagnetic chromium in multiple mouse organs", TOX APPL PH, 170(1), 2001, pp. 56-62

Abstract

DNA single-strand breaks (and/or alkali-labile sites) induced by Cr(VI) were evaluated with the alkaline single cell gel electrophoresis (SCC) (Comet) assay in five organs (liver, kidney, spleen, lung, and brain) of male mice dosed with K2Cr2O7 (20 mg Cr/kg) by a single ip injection in vivo, and the formation of paramagnetic Cr(V) in these organs was investigated by electron spin resonance (ESR) spectrometry. Furthermore, the in vivo effects of deferoxamine (DFO), an iron chelator, and dimethylthiourea (DMTU), a hydroxyl radical scavenger, on the formation of Cr(V) and DNA strand breaks induced by the metal in the liver and kidney were examined. SCG assay detected DNA strand breaks were detected in the liver and kidney at 15 min and showedthat they were being repaired at 3 h after Cr(VI) injection. The ESR spectra of paramagnetic Cr(V) were also observed in the liver and kidney for 15 min to 24 h after Cr(VI) injection. In contrast, there were no significant levels of DNA strand breaks and Cr(V) in the spleen, lung, or brain. The pretreatment of mice with DFO reduced the formation of Cr(VI)-induced DNA strand breaks and Cr(V) complexes as well as the total contents of Cr in the liver and kidney at 15 min after the metal injection. In the case of the pretreatment with DMTU, DNA strand breaks induced by Cr(VI) were suppressed inthe liver and kidney at 15 min, without any influence on the levels of Cr(V) complexes and total Cr contents in the organs. The in vitro study showedthat DFO decreased the levels of Cr(V)-GSH complexes and Cr(V)-mediated hydroxyl radicals, while DMTU reduced only the levels of Cr(V)-mediated hydroxyl radicals without affecting the formation of Cr(V)-GSH complexes. These results demonstrated that the SCG assay may be useful for detecting DNA strand breaks and/or alkali-labile sites caused by Cr(VI) in vivo. The resultsalso indicated that the in vivo formation of hydroxyl radicals during the reduction of Cr(VI) may play an important role in the induction of the DNA strand breaks caused by this metal and implied that the levels of Cr(V) inside the cells may not always be related to the induction of DNA strand breaks. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:00:11