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Titolo:
CD63 associates with the alpha(IIb)beta(3) integrin-CD9 complex on the surface of activated platelets
Autore:
Israels, SJ; McMillan-Ward, EM; Easton, J; Robertson, C; McNicol, A;
Indirizzi:
Univ Manitoba, Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada Univ Manitoba Winnipeg MB Canada R3E 0V9 ol, Winnipeg, MB R3E 0V9, Canada Univ Manitoba, Dept Pediat, Winnipeg, MB R3T 2N2, Canada Univ Manitoba Winnipeg MB Canada R3T 2N2 at, Winnipeg, MB R3T 2N2, Canada Univ Manitoba, Dept Oral Biol, Winnipeg, MB, Canada Univ Manitoba Winnipeg MB Canada a, Dept Oral Biol, Winnipeg, MB, Canada
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 1, volume: 85, anno: 2001,
pagine: 134 - 141
SICI:
0340-6245(200101)85:1<134:CAWTAI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HERMANSKY-PUDLAK SYNDROME; MONOCLONAL-ANTIBODY; ENDOTHELIAL-CELLS; TYROSINE PHOSPHORYLATION; MEMBRANE GLYCOPROTEIN; SIGNAL-TRANSDUCTION; GRANULE MEMBRANES; MOLECULAR-CLONING; PLASMA-MEMBRANE; ANTIGEN ME491;
Keywords:
CD antigens; blood platelets; platelet glycoprotein IIb/IIIa complex; platelet activation; cell membrane;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Israels, SJ Univ Manitoba, Manitoba Inst Cell Biol, 100 Olivia St, Winnipeg, MB R3E 0V9, Canada Univ Manitoba 100 Olivia St Winnipeg MB Canada R3E 0V9 Canada
Citazione:
S.J. Israels et al., "CD63 associates with the alpha(IIb)beta(3) integrin-CD9 complex on the surface of activated platelets", THROMB HAEM, 85(1), 2001, pp. 134-141

Abstract

The tetraspanins an integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and have been identified in multi-molecular complexes with specific integrins. In resting platelets, CD63, a member of the tetraspanin superfamily, is present in dense granule and lysosomal membranes and, following platelet activation, translocates to theplasma membrane. In the present study, platelet activation by thrombin leads to incorporation of CD63 into the Triton-insoluble actin cytoskeletal fraction. This incorporation was inhibited by preincubation of platelets withRGDS or EGTA and did not occur in platelets from a patient with Glanzmann's thrombasthenia, suggesting that it was dependent upon alpha (IIb)beta (3). In activated platelets, the anti-CD63 MoAb, D545, co-immunoprecipitated CD63 with other surface-labeled proteins, including alpha (IIb)beta (3) and another tetraspanin, CD9. The association of CD63 with CD9 and alpha (IIb)beta (3) was not inhibited by preincubation of platelets with RGDS or EGTA. D545 did not inhibit the adhesion of activated platelets to purified extracellular matrix proteins, but significantly decreased adhesion of thrombin-activated platelets to neutrophils in a resetting assay. D545 also caused disaggregation of platelets stimulated by ADP, but had no effect on aggregation induced by other agonists. These results are consistent with the proposal that CD63 becomes part of an alpha (IIb)beta (3)-CD9-CD63 integrin-tetraspanin complex in activated platelets - an association that may modulate thefunction of ol,alpha (IIb)beta (3)-dependent interaction with other cells such as neutrophils.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:16:09