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Titolo:
Double-stranded RNA interferes with gene function at the single-cell levelin cereals
Autore:
Schweizer, P; Pokorny, J; Schulze-Lefert, P; Dudler, R;
Indirizzi:
Max Planck Inst Zuchtungsforsch, D-50829 Cologne, Germany Max Planck Inst Zuchtungsforsch Cologne Germany D-50829 Cologne, Germany Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8008 Biol, CH-8008 Zurich, Switzerland
Titolo Testata:
PLANT JOURNAL
fascicolo: 6, volume: 24, anno: 2000,
pagine: 895 - 903
SICI:
0960-7412(200012)24:6<895:DRIWGF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSIENT EXPRESSION SYSTEM; VIRUS-RESISTANCE; CO-SUPPRESSION; ANTISENSE RNA; PETUNIA; PLANTS; DEGRADATION; TURNOVER; WHEAT;
Keywords:
Blumeria graminis; defence-related; Mlo; anthocyanin; barley; wheat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Schweizer, P Inst Plant Genet & Crop Plant Res IPK, Genome Ctr, D-06466 Gatersleben, Germany Inst Plant Genet & Crop Plant Res IPK Gatersleben Germany D-06466
Citazione:
P. Schweizer et al., "Double-stranded RNA interferes with gene function at the single-cell levelin cereals", PLANT J, 24(6), 2000, pp. 895-903

Abstract

Double-stranded RNA (dsRNA) has been shown to specifically interfere with gene function in several organisms including tobacco and the model plant Arabidopsis. Here, we report on rapid and sequence-specific interference of dsRNA with gene function in cereals. Delivery of cognate dsRNA into single epidermal cells of maize, barley or wheat by particle bombardment interferedwith the function of co-bombarded UidA (GUS) and TaGLP2a::GFP reporter genes. Cognate dsRNA was also found to specifically interfere with the function of the endogenous genes A1 and Ant18 encoding dihydroflavonol-4-reductasein maize and barley, respectively. Dihydroflavonol-4-reductase is an essential enzyme of the anthocyanin biosynthetic pathway in maize and barley. This pathway can be induced by transient expression of the C1- and 6-Peru genes that encode transcription factors. In the presence of dsRNA corresponding to the dihydroflavonol-4-reductase gene, C1- and b-Peru-dependent, cell-autonomous accumulation of red anthocyanin pigments in bombarded cells of maize and barley was reduced. dsRNA was also demonstrated to negatively interfere with Mlo, which encodes a negative regulator of race non-specific resistance to the powdery mildew fungus in barley. In the presence of Mlo dsRNA, transformed cells became more resistant, thereby phenocopying plants thatcarry a heritable loss-of function mlo resistance allele. The results suggest that direct delivery of dsRNA to cereals leads to a rapid and sequence-specific interference with gene function at the single-cell level.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 19:35:56