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Titolo:
Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growthof Rehmannia glutinosa plantlets in vitro
Autore:
Cui, YY; Hahn, EJ; Kozai, T; Paek, KY;
Indirizzi:
Chungbuk Natl Univ, Res Ctr Dev Adv Hort Technol, Cheonju 361763, South Korea Chungbuk Natl Univ Cheonju South Korea 361763 heonju 361763, South Korea Chiba Univ, Fac Hort, Matsudo, Chiba 2718510, Japan Chiba Univ Matsudo Chiba Japan 2718510 ort, Matsudo, Chiba 2718510, Japan
Titolo Testata:
PLANT CELL TISSUE AND ORGAN CULTURE
fascicolo: 3, volume: 62, anno: 2000,
pagine: 219 - 226
SICI:
0167-6857(2000)62:3<219:NOAESC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
POTATO PLANTLETS; IN-VITRO; MORPHOLOGY; SEEDLINGS; XHYBRIDA; CULTURE; INVITRO;
Keywords:
acclimatization; CO2 concentration; photosynthesis; reducing sugar; total sugar;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Paek, KY Chungbuk Natl Univ, Res Ctr Dev Adv Hort Technol, Cheonju 361763,South Korea Chungbuk Natl Univ Cheonju South Korea 361763 1763, South Korea
Citazione:
Y.Y. Cui et al., "Number of air exchanges, sucrose concentration, photosynthetic photon flux, and differences in photoperiod and dark period temperatures affect growthof Rehmannia glutinosa plantlets in vitro", PL CELL TIS, 62(3), 2000, pp. 219-226

Abstract

Rehmannia glutinosa plantlets were cultured for 4 weeks under different culture conditions to determine the optimum environment for in vitro growth and ex vitro survival. Plantlet growth increased with an increasing number of air exchanges of the culture vessel, exhibiting greatest shoot weight, total fresh weight, leaf area, and chlorophyll content at 4.4 h(-1) of air exchanges. High sucrose concentration (30 g l(-1)) increased root weight but reduced shoot growth. Net photosynthetic rates of the plantlets were greatest when sucrose was not added to the medium. On the other hand, ex vitro survival of the plantlets was not influenced by sucrose concentration. In theexperiment on difference in photoperiod and dark period temperatures (DIF)and photosynthetic photon flux (PPF), plantlet growth increased as DIF andPPF levels increased. Particularly, increasing PPF level had a more distinctive effect on plantlet growth than increasing DIF level. The interaction of DIF x PPF was also significant, showing the greatest plantlet growth in positive DIF (+8 DIF) and a high PPF (210 mu mol m(-2) s(-1)). In conclusion, the results of this experiment suggest that increased number of air exchanges of the culture vessel, decreased sucrose concentration, and positive DIF in combination with high PPF level enhanced growth and acclimatization of Rehmannia glutinosa plantlets.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:13:31