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Titolo:
Insecticidal activity of glufosinate through glutamine depletion in a caterpillar
Autore:
Kutlesa, NJ; Caveney, S;
Indirizzi:
Univ Western Ontario, Dept Zool, London, ON N6A 5B7, Canada Univ Western Ontario London ON Canada N6A 5B7 London, ON N6A 5B7, Canada
Titolo Testata:
PEST MANAGEMENT SCIENCE
fascicolo: 1, volume: 57, anno: 2001,
pagine: 25 -
SICI:
1526-498X(200101)57:1<25:IAOGTG>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOMBYX-MORI; AMMONIUM; SYNTHETASE; SILKWORM; PHOSPHINOTHRICIN; PURIFICATION; TOXICITY;
Keywords:
glufosinate; glutamine synthetase; glutamine; Calpodes ethlius; ammonia toxicity; feeding deterrent;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Caveney, S Univ Western Ontario, Dept Zool, London, ON N6A 5B7, Canada Univ Western Ontario London ON Canada N6A 5B7 N6A 5B7, Canada
Citazione:
N.J. Kutlesa e S. Caveney, "Insecticidal activity of glufosinate through glutamine depletion in a caterpillar", PEST MAN SC, 57(1), 2001, pp. 25

Abstract

The herbicide glufosinate-ammonium (GLA) is a competitive inhibitor of glutamine synthetase (GS), an enzyme converting glutamate to glutamine in bothplants and animals. Because GS is essential for ammonia detoxification in plants, GLA treatment disrupts photorespiration by causing a build-up of ammonia and a loss of glutamine in plant tissues. This study reports that GLAapplied to leaf surfaces is also toxic to 5th-instar caterpillars of the skipper butterfly Calpodes ethlius (LD50 = 400 mg kg(-1)). After ingesting GLA, caterpillars stopped feeding and became dehydrated through a loss of rectal function. Caterpillars showed symptoms of neurotoxicity, such as proleg tremors, body convulsions and complete paralysis before death. Incubationof several tissues isolated from normal feeding-stage caterpillars with the GS substrates glutamate and ammonium showed that GLA inhibited GS activity in vitro. Within 24 h of ingesting GLA, caterpillars had a greatly reduced glutamine content and the ammonium ion levels had more than doubled. Injection of ammonium chloride into non-GLA-treated caterpillars had no deleterious effect, suggesting that glutamine depletion, and not a rise in body ammonium, was the primary cause of GLA toxicity following GS inhibition. Thiswas supported by the observation that the onset of the symptoms of GLA poisoning could be postponed by giving GLA-fed caterpillars several subsequentdaily injections of glutamine. The effective GLA dose fed to 5th-instar caterpillars in this study was comparable to the amount that might realistically be acquired from feeding on GLA-treated crops. (C) 2001 Society of Chemical Industry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 15:53:28