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Titolo:
Involvement of endogenous PACAP expression in the activity-dependent survival of mouse cerebellar granule cells
Autore:
Tabuchi, A; Koizumi, M; Nakatsubo, J; Yaguchi, T; Tsuda, M;
Indirizzi:
Toyama Med & Pharmaceut Univ, Fac Pharmaceut Sci, Dept Biol Chem, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ Toyama Japan 9300194 Toyama 9300194, Japan Japan Sci & Technol Corp, Tokyo 1500002, Japan Japan Sci & Technol Corp Tokyo Japan 1500002 Corp, Tokyo 1500002, Japan
Titolo Testata:
NEUROSCIENCE RESEARCH
fascicolo: 1, volume: 39, anno: 2001,
pagine: 85 - 93
SICI:
0168-0102(200101)39:1<85:IOEPEI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLASE-ACTIVATING POLYPEPTIDE; ADENYLATE-CYCLASE; RAT-BRAIN; TISSUE DISTRIBUTION; GENE-EXPRESSION; KINASE PATHWAY; PROTEIN-KINASE; PITUITARY; NEURONS; BDNF;
Keywords:
PACAP; gene expression; calcium; cerebellar granule cells; apoptosis; cell survival; Ca2+ channel;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuda, M Toyama Med & Pharmaceut Univ, Fac Pharmaceut Sci, Dept Biol Chem,Sugitani2630, Toyama 9300194, Japan Toyama Med & Pharmaceut Univ Sugitani 2630 Toyama Japan 9300194
Citazione:
A. Tabuchi et al., "Involvement of endogenous PACAP expression in the activity-dependent survival of mouse cerebellar granule cells", NEUROSCI RE, 39(1), 2001, pp. 85-93

Abstract

Membrane depolarization causes Ca2+ influx through L-type voltage-dependent calcium channels (L-VDCC), which promotes the activity-dependent survivalof mouse cerebellar granule cells (CGCs). Although exogenously added pituitary adenylate cyclase activating polypeptide (PACAP) is effective: in promoting the survival of CGCs, it is unknown whether PACAP is synthesized in CGCs and involved in the activity-dependent survival of CGCs. In this study.we found that the PACAP gene was activated in depolarized CGCs cultured at25 mM KC1 thigh K+). independently of de novo protein synthesis. In addition, the PACAP immunoreactivity increased through the activation of L-VDCC in depolarized CGCs, indicating that PACAP is concomitantly produced with PACAP mRNA in an activity-dependent manner. Exogenously added PACAP attenuated the apoptosis of CGCs through a specific interaction with PACAP receptors. Furthermore, a PACAP receptor antagonist. PACAP(6-38), reduced the survival of CGCs at high K-1. These findings indicate that endogenous PACAP production induced by Ca signals exerts a survival effect on CGCs via PACAP receptors, which. at least in part, accounts for the activity-dependent survival of CGCs. (C) 2001 Published by Elsevier Science Ireland Ltd and the JapanNeuroscience Society. All rights reserved..

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Documento generato il 01/04/20 alle ore 11:36:04