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Titolo:
Nerve growth factor and glial cell line-derived neurotrophic factor restore the cholinergic neuronal phenotype in organotypic brain slices of the basal nucleus of Meynert
Autore:
Weis, C; Marksteiner, J; Humpel, C;
Indirizzi:
Univ Innsbruck Hosp, Dept Psychiat, Lab Psychiat, A-6020 Innsbruck, Austria Univ Innsbruck Hosp Innsbruck Austria A-6020 , A-6020 Innsbruck, Austria
Titolo Testata:
NEUROSCIENCE
fascicolo: 1, volume: 102, anno: 2001,
pagine: 129 - 138
SICI:
0306-4522(2001)102:1<129:NGFAGC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECEPTOR TYROSINE KINASE; WITHDRAWAL IN-VITRO; RET RECEPTOR; ALZHEIMERS-DISEASE; FOREBRAIN NEURONS; APOPTOTIC DEATH; MESSENGER-RNAS; GDNF; NGF; EXPRESSION;
Keywords:
NGF; GDNF; choline acetyltransferase; cholinergic phenotype; organotypic slice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Humpel, C Univ Innsbruck Hosp, Dept Psychiat, Lab Psychiat, Anichstr 35, A-6020 Innsbruck, Austria Univ Innsbruck Hosp Anichstr 35 Innsbruck AustriaA-6020 ustria
Citazione:
C. Weis et al., "Nerve growth factor and glial cell line-derived neurotrophic factor restore the cholinergic neuronal phenotype in organotypic brain slices of the basal nucleus of Meynert", NEUROSCIENC, 102(1), 2001, pp. 129-138

Abstract

Loss of cholinergic neurons is found in the medial septum and nucleus basalis of Meynert in Alzheimer's disease. Recent observations suggest that cholinergic neurons down-regulate their phenotype and that growth factors may rescue cholinergic neurons. The aim of this study was to investigate whether cholinergic neurons of the basal nucleus of Meynert can be cultured in rat organotypic slices, and if nerve growth factor and glial cell line-derived neurotrophic factor can rescue the cholinergic phenotype. In the organotypic slices, glial cells, GABAergic and cholinergic neurons were visualized using immunohistochemistry. The number of cholinergic neurons was found to be very low in slices cultured without exogenous nerve growth factor. Analysis of nerve growth factor tissue levels by enzyme-linked immunosorbent assay revealed very low endogenous tissue levels. When slices were incubated with 100 ng/ml nerve growth factor during the initial phase of culturing, a stable expression of choline acetyltransferase was found for up to several weeks. After eight weeks in culture with nerve growth factor or two to three weeks after nerve growth factor withdrawal, numbers of detected cholinergic neurons decreased. Neurons incubated with nerve growth factor displayed a significantly enlarged cell soma compared to neurons without growth factors. In cultures incubated for up to nine weeks, it was also found that glial cell line-derived neurotrophic factor was capable of restoring the cholinergic phenotype. The low-affinity p75 and high-affinity trkA receptors, as well as the glial cell line-derived neurotrophic factor receptor GFR alpha -1, could be visualized in slices using immunohistochemistry. In conclusion, it is shown that, in the axotomized organotypic slice model, the number of cholinergic neurons is decreased, but can be partly restored by nerve growth factor and glial cell line-derived neurotrophic factor. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 29/01/20 alle ore 18:53:46