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Titolo:
Changes of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate receptorsin layer V of epileptogenic, chronically isolated rat neocortex
Autore:
Kharazia, VN; Prince, DA;
Indirizzi:
Stanford Univ, Dept Neurol & Neurol Sci, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 & Neurol Sci, Stanford, CA 94305 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 1, volume: 102, anno: 2001,
pagine: 23 - 34
SICI:
0306-4522(2001)102:1<23:COAR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TEMPORAL-LOBE EPILEPSY; METHYL-D-ASPARTATE; SOMATIC SENSORY CORTEX; NEURONS IN-VIVO; GLUTAMATE RECEPTORS; CORTICOSPINAL NEURONS; PROJECTION NEURONS; CEREBRAL-CORTEX; FASCIA-DENTATA; VISUAL-CORTEX;
Keywords:
injury; axotomy; glutamate; NMDA; epilepsy; pyramidal neurons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Prince, DA Stanford Univ, Dept Neurol & Neurol Sci, Stanford, CA 94305 USAStanford Univ Stanford CA USA 94305 ci, Stanford, CA 94305 USA
Citazione:
V.N. Kharazia e D.A. Prince, "Changes of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate receptorsin layer V of epileptogenic, chronically isolated rat neocortex", NEUROSCIENC, 102(1), 2001, pp. 23-34

Abstract

In vivo chronic partial isolation of neocortical islands results in epileptogenesis that involves pyramidal neurons of layer V. To test whether an alteration in glutamate receptors might contribute to the epileptiform activity, we analysed the time-course of Light microscopic changes in expression of alpha -amino-3-hydroxy-5-methyl-4-isoxazole propionate receptors using subunit-specific antibodies. The isolation caused a rapid down-regulation ofimmunoreactivity for GluR1 and GluR2/3 subunits in deep layer V pyramidal neurons within the neocortical island which was evident 24 h post-lesion, and within three days was reduced to about 40-60% of the control level. Manypyramidal cells in deep layer V completely lacked GluR2. Between one and four weeks of survival, down-regulation of GluR2/3 and GluR2 involved the majority of pyramidal layer V neurons, except for cells in the upper pan of layer V, and those within narrow areas of all sub-laminae of layer V ("micro-islands"). Initial down-regulation was also observed one to three days post-lesion for subunits 1 and 2 of the N-methyl-D-aspartate receptor, but in contrast to GluR2/3 immunoreactivity, NMDAR2A/B immunoreactivity was enhanced three weeks post-lesion. The present data provide evidence for plastic changes in glutamate receptors in neurons of partially isolated neocortical island. A sub-population oflayer V neurons remains relatively unaffected, and would presumably be capable of generating fast glutamatergic synaptic potentials necessary for thedevelopment of synchronous epileptiform activity. (C) 2001 IBRO. Publishedby Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 02:14:44