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Titolo:
DOPAMINE-DENERVATION ENHANCES THE TROPHIC ACTIVITY IN STRIATUM - EVALUATION BY MORPHOLOGICAL AND ELECTROPHYSIOLOGICAL DEVELOPMENT IN PC 12DCELLS
Autore:
HIDA H; FUKUDA A; FUJIMOTO I; SHIMANO Y; NAKAJIMA K; HASHITANI T; NISHINO H;
Indirizzi:
NAGOYA CITY UNIV,SCH MED,DEPT PHYSIOL,MIUHO KU,KAWASUMI 1,NIZUHO CHO NAGOYA AICHI 467 JAPAN NAGOYA CITY UNIV,SCH MED,DEPT PHYSIOL,MIUHO KU NAGOYA AICHI 467 JAPAN
Titolo Testata:
Neuroscience research
fascicolo: 3, volume: 28, anno: 1997,
pagine: 209 - 221
SICI:
0168-0102(1997)28:3<209:DETTAI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; RAT PHEOCHROMOCYTOMA CELLS; SODIUM-CHANNEL EXPRESSION; VOLTAGE-DEPENDENT CALCIUM; SIGNAL-TRANSDUCTION; NEURITE OUTGROWTH; SPINAL NEURONS; K+ CHANNELS; PC12D CELLS; CURRENTS;
Keywords:
TROPHIC FACTOR; DOPAMINE; STRIATUM; DIFFERENTIATION; PC12D CELLS; PATCH CLAMP; CATION CHANNEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
H. Hida et al., "DOPAMINE-DENERVATION ENHANCES THE TROPHIC ACTIVITY IN STRIATUM - EVALUATION BY MORPHOLOGICAL AND ELECTROPHYSIOLOGICAL DEVELOPMENT IN PC 12DCELLS", Neuroscience research, 28(3), 1997, pp. 209-221

Abstract

To evaluate the possibility that dopamine (DA) denervation enhances the trophic activity in striatum, normal or DA-depleted striatal tissueextract (N- or L-extract, respectively) was obtained, and their trophic effects on PC12D cells were investigated from the viewpoints of differentiation using morphological and electrophysiological analyses. Treatment with N- or L-extract induced neurite outgrowth in a concentration-dependent manner, and induced the enlargement of cell size. These effects were stronger in L-extract than in N-extract. Cation currents were investigated in whole cell patch-clamp mode. Development of cation current started with delayed-rectifier type K+ current (I-K) and transient type K+ current (I-A), followed by Ca2+ current (I-Ca) and tetrodotoxin-sensitive Na+ current (I-Na). I-Na was expressed more frequently in L-extract treated cells than N-extract treated cells at D7-9. The larger I-K amplitude in L-extract treatment at D7-9 seemed to be related to the expression of I-Na. Development of I-A was similar at anystage for both treatments. I-Ca development started at D3-5 after treatments, and the amplitude and current density were similar in both treatments. I-Ca was strongly blocked by omega-conotoxin GVIA (3 mu M), indicating that N-type channels were mainly expressed after treatments. The data suggests that L-extract has stronger effects to hasten the differentiation of PC12D cells than N-extract by promoting the neuriteoutgrowth, cell enlargement and expression of voltage-dependent cation channels, especially I-Na and I-K. (C) 1997 Elsevier Science IrelandLtd.

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Documento generato il 21/09/20 alle ore 17:37:49