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Titolo:
Gene transfer methods for CNS organotypic cultures: A comparison of three nonviral methods
Autore:
Murphy, RC; Messer, A;
Indirizzi:
New York State Dept Hlth, Wadsworth Ctr Labs & Res, Albany, NY 12201 USA New York State Dept Hlth Albany NY USA 12201 & Res, Albany, NY 12201 USA SUNY Albany, Dept Biomed Sci, Albany, NY 12201 USA SUNY Albany Albany NY USA 12201 ny, Dept Biomed Sci, Albany, NY 12201 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 1, volume: 3, anno: 2001,
pagine: 113 - 121
SICI:
1525-0016(200101)3:1<113:GTMFCO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; MAMMALIAN-CELLS; EXPRESSION; DNA; ELECTROPORATION; MECHANISM; DELIVERY; NEURONS; BRAIN;
Keywords:
organotypic; electroporation; lipotransfection; biolistics; gene transfer; cerebellum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Messer, A New York State Dept Hlth, Wadsworth Ctr Labs & Res, Box 22002, Albany, NY 12201 USA New York State Dept Hlth Box 22002 Albany NY USA 12201 12201 USA
Citazione:
R.C. Murphy e A. Messer, "Gene transfer methods for CNS organotypic cultures: A comparison of three nonviral methods", MOL THER, 3(1), 2001, pp. 113-121

Abstract

Organotypic slice cultures from postnatal day 12 mouse cerebellum were transfected using three nonviral methods: biolistics (gene gun), lipotransfection, and electroporation. The plasmid transferred, pHD17-25Q-GFP, encoded afusion protein with a green fluorescent protein (GFP) component. Optimal conditions for both lipotransfection and electroporation are the same as those previously found in live animal models. Electroporation (26 +/- 6) and biolistics (34 +/- 4.4) provide a better rate of transfer than lipotransfection (15 +/- 2.2) in slice cultures and are comparable to each other. Each of the transfer methods produced positive signals in a heterogeneous population of glial and neuronal cells. These data provide a base for optimal transfection of slice cultures, allowing the development of therapeutic constructs, and support the idea that successful refinement of nonviral delivery methods for in vivo use is possible using brain slice cultures.

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Documento generato il 31/03/20 alle ore 15:34:34