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Titolo:
Generation of a stable cell line producing high-titer self-inactivating lentiviral vectors
Autore:
Xu, KL; Ma, H; McCown, TJ; Verma, IM; Kafri, T;
Indirizzi:
Univ N Carolina, Gene Therapy Ctr, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 y Ctr, Chapel Hill, NC 27599 USA Univ N Carolina, Dept Psychiat, Chapel Hill, NC 27599 USA Univ N CarolinaChapel Hill NC USA 27599 chiat, Chapel Hill, NC 27599 USA Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 munol, Chapel Hill, NC 27599 USA Salk Inst, Genet Lab, La Jolla, CA 92307 USA Salk Inst La Jolla CA USA 92307 k Inst, Genet Lab, La Jolla, CA 92307 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 1, volume: 3, anno: 2001,
pagine: 97 - 104
SICI:
1525-0016(200101)3:1<97:GOASCL>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIVO GENE DELIVERY; RETROVIRAL VECTORS; MAMMALIAN-CELLS; EXPRESSION; ONCOGENE; VIRUS;
Keywords:
lentiviral vectors; transduction; expression; neurons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Kafri, T Univ N Carolina, Gene Therapy Ctr, 7119 Thurston Bowles,CB 7352, Chapel Hill, NC 27599 USA Univ N Carolina 7119 Thurston Bowles,CB 7352 Chapel Hill NC USA 27599
Citazione:
K.L. Xu et al., "Generation of a stable cell line producing high-titer self-inactivating lentiviral vectors", MOL THER, 3(1), 2001, pp. 97-104

Abstract

To facilitate the generation of SIN lentivirus vector-producer cell lines,we have developed a novel conditional SIN (cSIN) lentivirus vector, which retains its SIN properties in normal target cells yet can be produced at high titers from tetracycline-regulated packaging cell lines. The design of the cSIN vector is based on replacing the vector U3 transcription regulatoryelements with the Tet-responsive element, which allows vector production exclusively in cells expressing the synthetic Tet-regulated transactivator (tTA). In contrast minimal vector production (similar to 200 IU/ml) is obtained in target cells that do not express the tTA, even in the presence of all HIV-1 proteins. Following transduction of the Tet-regulated SODk1 lentivirus vector-packaging cell line with the cSIN vector, high titers of cSIN recombinant vector (>10(6) IU/ml) could be generated, which efficiently transduced terminally differentiated neurons in normal rat brain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/08/20 alle ore 15:08:45