Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Selective transduction of murine myelomonocytic leukemia cells (WEHI-3B) with regular and RGD-adenoviral vectors
Autore:
Garcia-Castro, J; Segovia, JC; Garcia-Sanchez, F; Lillo, R; Gomez-Navarro, J; Curiel, DT; Bueren, JA;
Indirizzi:
CIEMAT, Unidad Biol Mol & Celular, Programa Terapia Gen, E-28040 Madrid, Spain CIEMAT Madrid Spain E-28040 Programa Terapia Gen, E-28040 Madrid, Spain Fdn Marcelino Botin, E-28040 Madrid, Spain Fdn Marcelino Botin Madrid Spain E-28040 no Botin, E-28040 Madrid, Spain Ctr Transfus Comunidad Madrid, Madrid 28009, Spain Ctr Transfus Comunidad Madrid Madrid Spain 28009 id, Madrid 28009, Spain Univ Alabama, Dept Pathol, Dept Med, Div Human Gene Therapy, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 ne Therapy, Birmingham, AL 35294 USA Univ Alabama, Dept Surg, Birmingham, AL 35294 USA Univ Alabama BirminghamAL USA 35294 Dept Surg, Birmingham, AL 35294 USA Univ Alabama, Gene Therapy Ctr, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 herapy Ctr, Birmingham, AL 35294 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 1, volume: 3, anno: 2001,
pagine: 70 - 77
SICI:
1525-0016(200101)3:1<70:STOMML>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEMATOPOIETIC-CELLS; MODIFIED FIBER PROTEIN; COXSACKIE-B VIRUSES; BREAST-CANCER CELLS; BONE-MARROW CELLS; GENE-TRANSFER; RECOMBINANT ADENOVIRUS; TRANSGENE EXPRESSION; MYELOMA CELLS; IN-VITRO;
Keywords:
gene therapy; adenoviral vectors; leukemia; RGD-adenoviruses; bone marrow purging; cancer cell targeting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Bueren, JA CIEMAT, Unidad Biol Mol & Celular, Programa Terapia Gen, E-28040 Madrid, Spain CIEMAT Madrid Spain E-28040 erapia Gen, E-28040 Madrid, Spain
Citazione:
J. Garcia-Castro et al., "Selective transduction of murine myelomonocytic leukemia cells (WEHI-3B) with regular and RGD-adenoviral vectors", MOL THER, 3(1), 2001, pp. 70-77

Abstract

On the basis of the susceptibility of normal myelomonocytic cells to adenoviral vectors, we have studied the possibility of selectively transducing myelomonocytic murine leukemic cells (WEHI-3B) with regular (Reg-Ad) and genetically modified (RCD-Ad) adenoviral vectors. An 8-h incubation of WEHI-3Bcells with 100 pfu of Reg-Ad vectors/cell resulted in the whole populationbecoming positive for transgene expression. Under identical conditions of infection, 20-30% of mouse bone marrow (BM) cells were positive for the transgene. When RCD-Ad vectors were used, a brief exposure (10 min) of WEHI-3Bcells to 150 pfu of the virus/cell was enough for 100% of the leukemia cells to become positive for the marker transgene (EGFP). Under these conditions, only 15-20% of BM cells and of primitive hematopoietic progenitors (Lin(-)Sca-(1+) cells) became EGFP(+), indicating an improved selectivity of the vectors for the leukemic cells. The incubation of WEHI-3B but not normal BM cells with soluble fiber protein (FP) inhibited the infection with Reg-Ad. The use of the RGD-Ad bypassed the FP-CAR interaction required for the transduction of WEHI-3B cells with Reg-Ad, suggesting that the abrogation ofthis requirement accounts for the improved infectivity of these leukemic cells and for the selectivity of RCD-Ad in targeting WEHI-3B leukemia cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 15:42:38