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Titolo:
Resistance of pancreatic carcinoma cells is reversed by coculturing NK-like T cells with dendritic cells pulsed with tumor-derived RNA and CA 19-9
Autore:
Ziske, C; Marten, A; Schottker, B; Buttgereit, P; Schakowski, F; Gorschluter, M; von Rucker, A; Scheffold, C; Chao, N; Sauerbruch, T; Schmidt-Wolf, IGH;
Indirizzi:
Univ Bonn, Med Klin & Poliklin 1, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 Klin & Poliklin 1, D-53105 Bonn, Germany Univ Bonn, Inst Klin Biochem, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 Inst Klin Biochem, D-53105 Bonn, Germany Stanford Univ, Ctr Med, Bone Marrow Transplantat Program, Stanford, CA 94305 USA Stanford Univ Stanford CA USA 94305 antat Program, Stanford, CA 94305 USA Duke Univ, Bone Marrow Transplantat Program, Durham, NC 27706 USA Duke Univ Durham NC USA 27706 Transplantat Program, Durham, NC 27706 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 1, volume: 3, anno: 2001,
pagine: 54 - 60
SICI:
1525-0016(200101)3:1<54:ROPCCI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOLOGICAL EFFECTOR-CELLS; INDUCED KILLER-CELLS; MESSENGER-RNA; IN-VIVO; ANTITUMOR IMMUNITY; CYTO-TOXICITY; EXPRESSION; DIFFERENTIATION; IMMUNIZATION; INDUCTION;
Keywords:
pancreatic neoplasm; immunotherapy; RNA; dendritic cells; NK cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Schmidt-Wolf, IGH Univ Bonn, Med Klin & Poliklin 1, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 , D-53105 Bonn, Germany
Citazione:
C. Ziske et al., "Resistance of pancreatic carcinoma cells is reversed by coculturing NK-like T cells with dendritic cells pulsed with tumor-derived RNA and CA 19-9", MOL THER, 3(1), 2001, pp. 54-60

Abstract

Immunization with defined tumor antigens is limited to the small number ofcancers in which specific tumor antigens have been defined but insufficient tumor material is available to produce an antitumor vaccine. In this study, we investigated whether pulsing dendritic cells (DC) using a liposomal transfer technique with a pancreatic tumor cell line-derived RNA can effectively activate NK-like T cells and tumor immunity. Pulsed DC were coculturedwith NK-like T cells, i.e., CD3(+)CD56(+) cells, as immunologic: effector cells. Target cells resistant to NK-like T-cell-mediated lysis were used. Total tumor-derived RNA transfected into DC was found to completely reverse tumor cell resistance. Total tumor RNA transfection (30 mug) was found to be superior to poly(A)(+) RNA transfection (5 mug) in inducing NK-like T lymphocytes. Interestingly, additional pulsing of DC with the CA 19-9 peptide in a CA 19-9-positive cell line further increased the sensitivity of pancreas carcinoma cells to NK-like T cells. Treatment of tumor RNA with RNase completely blocked the effect of RNA-transfected DC on NK-like T cells, suggesting that intact tumor-derived RNA is needed for reversal of tumor cell resistance. In conclusion, coculture of NK-like T cells with DC transfected with pancreatic tumor cell line-derived RNA reverses pancreatic tumor cell resistance by directly triggering NK-like T lymphocytes.

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Documento generato il 25/01/20 alle ore 15:39:22