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Titolo:
Immunomodulation by electrically enhanced delivery of plasmid DNA encodingIL-12 to murine skeletal muscle
Autore:
Lucas, ML; Heller, R;
Indirizzi:
Univ S Florida, Dept Surg, Ctr Mol Delivery, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 , Ctr Mol Delivery, Tampa, FL 33612 USA Univ S Florida, Dept Med Microbiol & Immunol, Inst Biomol Sci, Tampa, FL 33612 USA Univ S Florida Tampa FL USA 33612 l, Inst Biomol Sci, Tampa, FL 33612 USA
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 1, volume: 3, anno: 2001,
pagine: 47 - 53
SICI:
1525-0016(200101)3:1<47:IBEEDO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO ELECTROPORATION; DIRECT GENE-TRANSFER; RAT-LIVER; ELECTROCHEMOTHERAPY; EXPRESSION; MELANOMA; THERAPY; INVIVO; CELLS;
Keywords:
electroporation; in vivo; muscle; luciferase; IL-12; IFN-gamma; immunotherapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Heller, R Univ S Florida, Dept Surg, Ctr Mol Delivery, MDC 16,12901 Bruce B Downs Blvd, Tampa, FL 33612 USA Univ S Florida MDC 16,12901 Bruce B DownsBlvd Tampa FL USA 33612
Citazione:
M.L. Lucas e R. Heller, "Immunomodulation by electrically enhanced delivery of plasmid DNA encodingIL-12 to murine skeletal muscle", MOL THER, 3(1), 2001, pp. 47-53

Abstract

Recent reports have shown the successful delivery of plasmid DNA to skeletal muscle by in vivo electroporation. However, most of these reports have used what has been characterized as low-voltage, millisecond pulses. One of the purposes of this study was to examine a wide range of parameters to establish a better understanding of the effects of field strength and pulse length. Two electroporation conditions, one utilizing low-voltage millisecondpulses and the other high-voltage microsecond pulses, were compared for their ability to deliver a molecule that can be measured systemically, IL-12. Not only was this molecule efficiently delivered, but one of the moleculesit induces (IFN-gamma) was also measured systemically, with expression peaking at greater than 200 pg/ml serum levels on day 7 and still as high as 40 pg/ml on day 21 when using electrical parameters of 100 V/cm, 20-ms pulses. Of the two types of parameters tested, low-voltage, millisecond pulses resulted in higher, prolonged expression of plasmid DNA than high-voltage, microsecond pulses. In summary, this report provides evidence that in vivo electroporation is an efficient method for the delivery of plasmid DNA, and its use in immunotherapy protocols should be furthered examined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:12:50