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Titolo:
A two-component system involving an HD-GYP domain protein links cell-cell signalling to pathogenicity gene expression in Xanthomonas campestris
Autore:
Slater, H; Alvarez-Morales, A; Barber, CE; Daniels, MJ; Dow, JM;
Indirizzi:
John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res Norwich Norfolk England NR4 7UH olk, England Univ Irapuato, Dept Plant Genet Engn, IPN, CINVESTAV, Irapuato 36500, GTO,Mexico Univ Irapuato Irapuato GTO Mexico 36500 ESTAV, Irapuato 36500, GTO,Mexico
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 5, volume: 38, anno: 2000,
pagine: 986 - 1003
SICI:
0950-382X(200012)38:5<986:ATSIAH>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GRAM-NEGATIVE BACTERIA; LUXR-LUXI FAMILY; PV CAMPESTRIS; VIBRIO-HARVEYI; BORDETELLA-PERTUSSIS; EXTRACELLULAR POLYSACCHARIDE; TRANSCRIPTIONAL REGULATORS; 2-COMPONENT PHOSPHORELAY; INSERTION MUTAGENESIS; PATHOVAR CAMPESTRIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Dow, JM John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res Norwich Res Pk Norwich Norfolk England NR4 7UH
Citazione:
H. Slater et al., "A two-component system involving an HD-GYP domain protein links cell-cell signalling to pathogenicity gene expression in Xanthomonas campestris", MOL MICROB, 38(5), 2000, pp. 986-1003

Abstract

The synthesis of extracellular enzymes and extracellular polysaccharide (EPS) in Xanthomonas campestris pv. campestris (Xcc) is regulated by a cluster of genes called rpf (for regulation of pathogenicity factors). Two of thegenes, rpfF and rpfB, have previously been implicated in the synthesis of a diffusible regulatory molecule, DSF. Here, we describe a screen of transposon insertion mutants of Xcc that identified two DSF-overproducing strains. In each mutant, the gene disrupted is rpfC, which encodes a hybrid two-component regulatory protein in which the sensor and regulator domains are fused and which contains an additional C-terminal phosphorelay (HPt) domain. We show that rpfC is in an operon with rpfH and rpfG. The predicted proteinRpfG has a regulatory input domain attached to a specialized version of anHD domain, previously suggested to function in signal transduction. The predicted protein RpfH is structurally related to the sensory input domain ofRpfC. We show that RpfC and RpfG act positively to regulate the synthesis of extracellular enzymes and EPS, but that RpfC acts negatively to regulatethe synthesis of DSF. We propose that RpfGHC is a signal transduction system that couples the synthesis of pathogenicity factors to sensing of environmental signals that may include DSF itself.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 15:04:02