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Titolo:
Expression profiles of interleukin-15 in early and late gestational human placenta and in pre-eclamptic placenta
Autore:
Agarwal, R; Loganath, A; Roy, AC; Wong, YC; Ng, SC;
Indirizzi:
Natl Univ Singapore, Natl Univ Hosp, Dept Obstet & Gynaecol, Singapore 119074, Singapore Natl Univ Singapore Singapore Singapore 119074 ngapore 119074, Singapore
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 1, volume: 7, anno: 2001,
pagine: 97 - 101
SICI:
1360-9947(200101)7:1<97:EPOIIE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; NORMAL HUMAN-PREGNANCY; HUMAN FETAL MEMBRANES; TROPHOBLAST INVASION; CYTOKINE PRODUCTION; PRETERM LABOR; IN-VITRO; CELLS; APOPTOSIS; RECEPTOR;
Keywords:
first trimester; IL-15; labour; placenta; pre-eclampsia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Loganath, A Natl Univ Singapore, Natl Univ Hosp, Dept Obstet & Gynaecol, Lower Kent Ridge Rd, Singapore 119074, Singapore Natl Univ Singapore Lower Kent Ridge Rd Singapore Singapore 119074
Citazione:
R. Agarwal et al., "Expression profiles of interleukin-15 in early and late gestational human placenta and in pre-eclamptic placenta", MOL HUM REP, 7(1), 2001, pp. 97-101

Abstract

The presence of interleukin-15 (IL-15) mRNA in human placenta has been demonstrated previously. The present study was undertaken to investigate the expression profiles of IL-15 mRNA and protein in early and late gestational placental tissues, and also the effect of labour on its production. Levels of placental IL-15 expression were also determined in patients presenting with pre-eclampsia. An explant culture system was used to study the release of immunoreactive IL-15 by the placental tissues. Enzyme-linked immunosorbent assays were employed to quantify concentrations in the culture medium. The results showed that placental tissues from all groups released immunoreactive IL-15 into the culture medium. Moreover, the level of secretion by the term placental tissues was much higher than that by first trimester tissues. The presence of labour at term resulted ina further increase in placental IL-15 production. Reverse transcription-polymerase chain reaction (RT-PCR) was used to demonstrate the expression of IL-15 mRNA in these tissues. The results confirmed the expression of IL-15 in placenta from all the groups and the mRNA levels in the samples was highly correlated with the respective protein concentrations. Levers of both IL-15 mRNA and protein were significantly reduced in the pre-eclamptic placental tissue compared with the normal controls. The present study suggests an important role for this novelcytokine in human pregnancy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:01:36