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Titolo:
Presence of antibodies to sperm YLP12 synthetic peptide in sera and seminal plasma of immunoinfertile men
Autore:
Naz, RK; Chauhan, SC;
Indirizzi:
Med Coll Ohio, Div Res, Dept Obstet & Gynecol, Toledo, OH 43614 USA Med Coll Ohio Toledo OH USA 43614 Obstet & Gynecol, Toledo, OH 43614 USA
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 1, volume: 7, anno: 2001,
pagine: 21 - 26
SICI:
1360-9947(200101)7:1<21:POATSY>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTISPERM ANTIBODIES; FERTILIZATION ANTIGEN; INFERTILITY; FA-1; SPERMATOZOA; INVOLVEMENT; PROTEINS;
Keywords:
antisperm antibodies; fertilization; infertility; semen; sperm antigens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Naz, RK Med Coll Ohio, Div Res, Dept Obstet & Gynecol, Hlth Educ Bldg,Room211,3055 Arlington Ave, Toledo, OH 43614 USA Med Coll Ohio Hlth Educ Bldg,Room 211,3055 Arlington Ave Toledo OH USA 43614
Citazione:
R.K. Naz e S.C. Chauhan, "Presence of antibodies to sperm YLP12 synthetic peptide in sera and seminal plasma of immunoinfertile men", MOL HUM REP, 7(1), 2001, pp. 21-26

Abstract

Using an enzyme-linked immunosorbent assay (ELISA), sera (n = 15) and seminal plasma (n = 30) from antisperm antibody-positive immunoinfertile men (n= 45) and from fertile men (n = 45), were tested for the immunoreactivity with the synthetic YLP12 sperm peptide, Of the 15 immunoinfertile sera tested, 46 % were positive for immunoglobulin (Tg)M, 73% for IgG, and 40% for IgA. Of the 30 samples of immunoinfertile seminal plasma tested, 10% were positive for IgM, 20% for IgG, and 43% for IgA, None of the sera or seminal plasma from fertile men showed a positive reaction. There was no significantcorrelation between the sperm immobilization technique (SIT) or tray agglutination technique (TAT) titres or percentage binding in immunobead bindingtechnique (IBT) and the antibody reactivity for any class in the ELISA. The YLP12 peptide conjugated to bovine serum albumin-Sepharose 4B beads pulled out IgG antibodies from the serum of the immunoinfertile, but not the fertile, men. The beads pulled out IgA antibodies from the immunoinfertile, but not the fertile, seminal plasma. The immune-affinity purified antipeptideantibodies reacted with a specific band of 72 +/- 5 kDa in the human testis and with a specific band of similar to 50 +/- 5 kDa in the human sperm extracts. The YLP12 peptide may have applications in the specific diagnosis and treatment of male infertility and in contraceptive vaccine development.

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Documento generato il 04/04/20 alle ore 01:19:54