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Titolo:
Separation-of-function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombination
Autore:
Qiu, JX; Kale, SB; Schultz, HY; Roth, DB;
Indirizzi:
Baylor Coll Med, Dept Immunol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Dept Immunol, Houston, TX 77030 USA Baylor Coll Med, Howard Hughes Med Inst, Houston, TX 77030 USA Baylor CollMed Houston TX USA 77030 ghes Med Inst, Houston, TX 77030 USA Baylor Coll Med, Interdisciplinary Program Cell & Mol Biol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ll & Mol Biol, Houston, TX 77030 USA
Titolo Testata:
MOLECULAR CELL
fascicolo: 1, volume: 7, anno: 2001,
pagine: 77 - 87
SICI:
1097-2765(200101)7:1<77:SMRCRF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEVERE COMBINED IMMUNODEFICIENCY; CELL RECEPTOR GAMMA; PRE-B CELLS; SIGNAL SEQUENCES; STRUCTURE REQUIREMENTS; JUNCTIONAL SEQUENCES; KU86-DEFICIENT MICE; DNA-SEQUENCE; SCID MICE; 2 STEPS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Roth, DB Baylor Coll Med, Dept Immunol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 unol, Houston, TX 77030 USA
Citazione:
J.X. Qiu et al., "Separation-of-function mutants reveal critical roles for RAG2 in both the cleavage and joining steps of V(D)J recombination", MOL CELL, 7(1), 2001, pp. 77-87

Abstract

The only established physiological function of the V(D)J recombinase, comprising RAG1 and RAG2, is to perform DNA cleavage. The molecular roles of RAG2 in cleavage, the mechanisms used to join the broken DNA ends, and the identity of nuclease(s) that open the hairpin coding ends have been unknown. Site-directed mutagenesis targeting each conserved basic amino acid in RAG2revealed several separation-of-function mutants that address these questions. Analysis of these mutants reveals that RAG2 helps recognize or cleave distorted DNA intermediates and plays an essential role in the joining step of V(D)J recombination. Moreover, the discovery that some mutants block RAG-mediated hairpin opening in vitro provides a critical link between this biochemical activity and coding joint formation in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:41:02