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Titolo:
Generation of polyclonal antiserum against the growth hormone secretagoguereceptor (GHS-R) - Evidence that the GHS-R exists in the hypothalamus, pituitary and stomach of rats
Autore:
Shuto, YJ; Shibasaki, T; Wada, K; Parhar, I; Kamegai, J; Sugihara, H; Oikawa, S; Wakabayashi, I;
Indirizzi:
Nippon Med Sch, Dept Med, Bunkyo Ku, Tokyo 1138603, Japan Nippon Med Sch Tokyo Japan 1138603 Med, Bunkyo Ku, Tokyo 1138603, Japan Nippon Med Sch, Dept Physiol, Bunkyo Ku, Tokyo 1138603, Japan Nippon Med Sch Tokyo Japan 1138603 siol, Bunkyo Ku, Tokyo 1138603, Japan
Titolo Testata:
LIFE SCIENCES
fascicolo: 9, volume: 68, anno: 2001,
pagine: 991 - 996
SICI:
0024-3205(20010119)68:9<991:GOPAAT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PEPTIDE; NEURONS;
Keywords:
growth hormone secretagogue receptor; ghrelin; antibody; rat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Shuto, YJ Nippon Med Sch, Dept Med, Bunkyo Ku, 1-1-5 Sendagi, Tokyo 1138603, Japan Nippon Med Sch 1-1-5 Sendagi Tokyo Japan 1138603 1138603, Japan
Citazione:
Y.J. Shuto et al., "Generation of polyclonal antiserum against the growth hormone secretagoguereceptor (GHS-R) - Evidence that the GHS-R exists in the hypothalamus, pituitary and stomach of rats", LIFE SCI, 68(9), 2001, pp. 991-996

Abstract

Growth hormone (GH) secretagogues (GHSs), which stimulate GH secretion, are synthetic compounds that act through the GHS receptor (GHS-R) which has been recently cloned. We raised an antiserum in a rabbit against a syntheticpeptide corresponding to amino acid residues 248-260 of the third intracellular loop of the rat GHS-R. A competitive immunoassay showed that the antiserum had a specific affinity for the target peptide. To confirm the specificity of the antiserum, the GHS-R cDNA was stably expressed in COS-7 cells. In Western blot analysis, the band was detected at 44 kDa in the extracts from COS-7 cells expressing GHS-R (COS-7/tf3-2) but not in those from wild-type COS-7 cells. Furthermore, while COS-7/tf3-2 cells were strongly immunostained for GHS-R, no GHS-R-like immunoreactivity was observed in wild-typeCOS-7 cells. Immunoreactive bands were also observed at approximately 46 kDa in the extracts from rat hypothalamus, pituitary and stomach by Western blot analysis. These studies are the first to show the existence of GHS-R protein in the stomach. The antiserum for the GHS-R is sensitive and specific, and it would be useful for clarifying the roles of GHS/ghrelin. (C) 2001Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:46:43