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Titolo:
Activation-induced cell death in peripheral blood mononuclear cells (PBMCs) from patients with chronic hepatitis B may be related to abnormal production of interleukin 12 and 10
Autore:
Ji, W; Wang, HF; Feng, CQ;
Indirizzi:
302 Hosp PLA, Dept Infect Dis 4, Beijing 100039, Peoples R China 302 Hosp PLA Beijing Peoples R China 100039 jing 100039, Peoples R China
Titolo Testata:
JOURNAL OF VIRAL HEPATITIS
fascicolo: 1, volume: 8, anno: 2001,
pagine: 30 - 33
SICI:
1352-0504(200101)8:1<30:ACDIPB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYPE-2 CYTOKINES; VIRAL CLEARANCE; T-LYMPHOCYTES; APOPTOSIS; INDUCTION; IMMUNITY; IL-12; TH1;
Keywords:
interleukin-12 heterodimer; interleukin-10; hepatitis B virus; chronic hepatitis B; activation-induced cell death;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Ji, W 302 Hosp PLA, Dept Infect Dis 4, Beijing 100039, Peoples R China 302Hosp PLA Beijing Peoples R China 100039 00039, Peoples R China
Citazione:
W. Ji et al., "Activation-induced cell death in peripheral blood mononuclear cells (PBMCs) from patients with chronic hepatitis B may be related to abnormal production of interleukin 12 and 10", J VIRAL HEP, 8(1), 2001, pp. 30-33

Abstract

Peripheral blood mononuclear cells (PBMCs) from 70 patients with chronic hepatitis B and 32 normal healthy persons were isolated and cultured with orwithout Staphylococcus aureus enterotoxin B (SEB; 0.2 mg l(-1)) and recombinant HBcAg (rHBcAg; 1.0 mg l(-1)) for 48 h in vitro. After incubation, thecells were harvested by centrifugation and apoptosis of the PBMCs was studied by staining with fluorescent dyes YOPRO-1 and Hoechst 33342. The levelsof IL-12 and IL-10 in the serum and the supernatants of cultured PBMCs were assayed by ELISA. The levels of IL-12 heterodimer in the serum and the supernatants of PBMCs cultured with SEB or rHBcAg were lower in patients thancontrols. The levels of IL-10 in both the serum and supernatants were higher in patients than controls. In addition, the percentage of apoptotic cells in PBMCs from the infected patients was significantly greater than from normal persons in the presence or absence of SEB and rHBcAg. Patients seropositive for HBeAg had much greater percentage of apoptotic cells in the PBMCs cultured with rHBcAg than patients seronegative for HBeAg, reaching 24.08%. We speculate that activation-induced cell death of PBMCs in the patientswith hepatitis B may be related to abnormal expression of IL-12 heterodimer and IL-10, which may lead to persistent infection in the patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:44:53