Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Evaluation of myelotoxicity in cotton rats (Sigmodon hispidus) exposed to environmental contaminants. I. In vitro bone-marrow progenitor culture
Autore:
Kim, S; Stair, EL; Lochmiller, RL; Lish, JW; Qualls, CW;
Indirizzi:
Oklahoma State Univ, Coll Vet Med, Dept Vet Pathobiol, Stillwater, OK 74078 USA Oklahoma State Univ Stillwater OK USA 74078 iol, Stillwater, OK 74078 USA Oklahoma State Univ, Dept Zool, Stillwater, OK 74078 USA Oklahoma State Univ Stillwater OK USA 74078 ool, Stillwater, OK 74078 USA Oklahoma State Univ, Coll Vet Med, Dept Physiol Sci, Stillwater, OK 74078 USA Oklahoma State Univ Stillwater OK USA 74078 Sci, Stillwater, OK 74078 USA
Titolo Testata:
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
fascicolo: 2, volume: 62, anno: 2001,
pagine: 83 - 96
SICI:
1528-7394(200101)62:2<83:EOMICR>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; 25 GROUNDWATER CONTAMINANTS; TRACHEAL EPITHELIAL-CELLS; CYCLOPHOSPHAMIDE EXPOSURE; ALVEOLAR MACROPHAGES; CONDITIONED MEDIUM; CHEMICAL-MIXTURE; B6C3F1 MICE; GM-CSF; GRANULOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Qualls, CW Glaxo Wellcome Inc, Med Safety Evaluat, 5 Moore Dr, Res Triangle Pk, NC 27709 USA Glaxo Wellcome Inc 5 Moore Dr Res Triangle Pk NC USA 27709 USA
Citazione:
S. Kim et al., "Evaluation of myelotoxicity in cotton rats (Sigmodon hispidus) exposed to environmental contaminants. I. In vitro bone-marrow progenitor culture", J TOX E H A, 62(2), 2001, pp. 83-96

Abstract

Bone marrow is extremely sensitive to toxicants, and in vitro culture of bone-marrow progenitor cells has been shown to be a sensitive indicator of bone-marrow injury in laboratory rodents. The ability of a bone-marrow progenitor cell assay to detect myelotoxicity in a wild rodent model ( cotton rat, Sigmodon hispidus) that inhabits many contaminated ecosystems in the southern United States was examined. Responsiveness of progenitor cells to recombinant murine granulocyte-macrophage colony-stimulating factor ( GM-CSF) and cotton rat lung-conditioned medium (LCM) was determined to optimize culture conditions for cotton rats. Myelotoxicity was induced in cotton rats by treating animals with either cyclophosphamide (8 or 80 mg/kg) or dexamethasone (500 mug/kg) over a 5-d period. Administration of a high dose of cyclophosphamide caused nearly total suppression of colony formation of granulocyte-macrophage progenitor cells ( CFU-GM). Marked histological changes in both the bone marrow and spleen were also observed in cotton rats treated with a high dose of cyclophosphamide. Although histological lesions were notapparent, the number of CFU-GM in the bone marrow of low-dose cyclophosphamide- and dexamethasone-treated cotton rats was significantly suppressed compared to controls. The number of CFU-GM was consistently higher using LCM than recombinant murine GM-CSF. This reproducible, quantitative, in vitro bone-marrow progenitor cell culture system was a sensitive indicator of myelotoxicity in wild cotton rats and should be useful for monitoring chronic exposures to low levels of environmental toxicants in wild rodent populations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 15:09:48